Publications Archive
Publications in 2022
Journal Articles (subsidised)
BRAGAN?A PHN, BELLSTEDT DU, VAN DER MERWE PDW, COTTERILL FPD, WATTERS BR, CHAKONA A. Nothobranchius balamaensis (Cyprinodontiformes: Nothobranchiidae), a new species of annual killifish from northern Mozambique. Zootaxa 2022; 5174(5):508-524.
CAILLET C, STOFBERG ML, MULEYA V, SHONHAI A, ZININGA T. Host cell stress response as a predictor of COVID-19 infectivity and disease progression. Frontiers in Molecular Biosciences 2022; 9:938099, 24 pages.
CARTWRIGHT M, LOUW-DU TOIT R, AFRICANDER DJ. The transcriptional activity of progestins used in contraception and menopausal hormone therapy via progesterone receptor A is dependent on the density of the receptor. Biochemical and Biophysical Research Communications 2022; 639:70-76.
CHAKAFANA G, MAKUMIRE S, ZININGA T, SHONHAI A. Exploring the application of ELISA and slot blot as utility protein–protein interaction analysis tools. Transactions of the Royal Society of South Africa 2022; 77(3):175-180.
CLEMENTS-DECKER T, RAUTENBACH M, KHAN S, KHAN W. Metabolomics and Genomics Approach for the Discovery of Serrawettin W2 Lipopeptides from Serratia marcescens NP2. JOURNAL OF NATURAL PRODUCTS 2022; 85:1256-1266.
KOMANE M, AVENANT C, LOUW-DU TOIT R, AFRICANDER DJ, HAPGOOD JP. Differential off-target glucocorticoid activity of progestins used in endocrine therapy. STEROIDS 2022; 182:108998, 9 pages.
KOTHMANN KH, JONS A, WILHELMI B, KASOZI N, GRAHAM L, GENT R, ATKIN SL, SWART AC, NEWELL-FUGATE AE. Non-invasive assessment of fecal glucocorticoid, progesterone, and androgen metabolites and microbiome in free-ranging southern white rhinoceros (Ceratotherium simum simum) in South Africa. GENERAL AND COMPARATIVE ENDOCRINOLOGY 2022; 329:114099, 13 pages.
KUSCHMIERZ L, SHEN L, BR?SEN C, SNOEP JL, SIEBERS B. Workflows for optimization of enzyme cascades and whole cell catalysis based on enzyme kinetic characterization and pathway modelling. CURRENT OPINION IN BIOTECHNOLOGY 2022; 74:55-60.
LAUBSCHER WE, RAUTENBACH M. Direct detection of antibacterial-producing soil isolates utilizing a novel high-throughput screening assay. Microorganisms 2022; 10:2235, 20 pages.
LEUSSA NA, RAUTENBACH M. Antiplasmodial cyclodecapeptides from tyrothricin share a target with chloroquine. Antibiotics 2022; 11:801, 22 pages.
LOMBARD N, STANDER MA, REDELINGHUYS H, LE ROUX MM, VAN WYK B-E. A study of phenolic compounds and their chemophenetic value in the genus Thesium (Santalaceae). Diversity 2022; 14:590, 28 pages.
LOOS B, DU TOIT A, HOFMEYR JS. Non-invasive monitoring of autophagy. Nature Biomedical Engineering 2022; 6:1015-1016.
MAGANGANA TP, MAKUNGA NP, FAWOLE OA, STANDER MA, OPARA UL. Antioxidant, Antimicrobial, and Metabolomic Characterization of Blanched Pomegranate Peel Extracts: Effect of Cultivar. Molecules 2022; 27:2979, 24pages.
MAKUNGA NP, SCHROEDER E, STANDER MA. Mesembrine alkaloid production in in vitro culture morphotypes of Sceletium tortuosum (L.) N.E. Br. Industrial Crops and Products 2022; 185:115051, 12pages.
MASIKE K, DE VILLIERS AJ, DE BEER D, JOUBERT E, STANDER MA. Application of direct injection-ion mobility spectrometry-mass spectrometry (DI-IMS-MS) for the analysis of phenolics in honeybush and rooibos tea samples. Journal of Food Composition and Analysis 2022; 106:104308: 11.
MUTHELO T, MULAUDZI V, NETSHISHIVHE M, DONGOLA TH, KOK M, MAKUMIRE S, DE VILLIERS M, BURGER A, ZININGA T, SHONHAI A. Inhibition of Plasmodium falciparum Hsp70-Hop partnership by 2-phenylthynesulfonamide. Frontiers in Molecular Biosciences 2022; 9:947203, 15 pages.
NGUBO M, REID JL, PATTERTON H. Distinct structural groups of histone H3 and H4 residues have divergent effects on chronological lifespan in Saccharomyces cerevisiae. PLoS One 2022; 17(5):e0268760; 23pp.
OLIVIER T, LOOTS L, KOK M, DE VILLIERS M, READER J, BIRKHOLTZ L, ARNOTT GE, DE VILLIERS K. Adsorption to the surface of hemozoin crystals: Structure-based design and synthesis of amino-phenoxazine β-hematin inhibitors. ChemMedChem 2022; 17(10):e202200139, 13 pages.
PERKINS MS, LOUW-DU TOIT R, JACKSON HC, SIMONS M, AFRICANDER DJ. Upregulation of an estrogen receptor-regulated gene by first generation progestins requires both the progesterone receptor and estrogen receptor alpha. Frontiers in Endocrinology 2022; 13:959396, 14 pages.
REDDY K, STANDER MA, STAFFORD GI, MAKUNGA NP. Mass Spectrometry Metabolomics and Feature-Based Molecular Networking Reveals Population-Specific Chemistry in Some Species of the Sceletium Genus. Frontiers in Nutrition 2022; 9:819753, 20 pages.
SCHIFFER L, SHAHEEN F, GILLIGAN L, STORBECK K, HAWLEY JS, KEEVIL BG, ARLT W, TAYLOR AE. Multi-steroid profiling by UHPLC-MS/MS with post-column infusion of ammonium fluoride. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences 2022; 1209:123413, 11 pages.
SCHMERLING C, KOURIL T, SNOEP JL, BR?SEN C, SIEBERS B. Enhanced underground metabolism challenges life at high temperature–metabolic thermoadaptation in hyperthermophilic Archaea. Current Opinion in Systems Biology 2022; 30:100423, 9 pages.
SHAIKH B, SMITH LP, VASILESCU D, MARUPILLA G, WILSON M, AGMON E, ROHWER JM, SNOEP JL, VAN NIEKERK DD, ET AL. BioSimulators: a central registry of simulation engines and services for recommending specific tools. Nucleic Acids Research 2022; 50:W108-W114.
SPEELMAN TS, DALE L, LOUW A, VERHOOG NJD. The association of acute phase proteins in stress and inflammation-induced T2D. Cells 2022; 11:2163, 20 pages.
SPIES LL, VERHOOG NJD, LOUW A. Relative contribution of molecular mechanisms to cumulative ligand-mediated downregulation of GRα. Biochemical and Biophysical Research Communications 2022; 602:113-119.
STORBECK K. A commentary on the origins of 11-ketotestosterone. European Journal of Endocrinology 2022; 187(6):C5-C8.
TIMMERMANS S, VERHOOG NJD, VAN LOOVEREN K, DEWAELE S, HOCHEPIED T, EGGERMONT M, GILBERT B, BOEREMA-DE MUNCK A, LOUW A, ET AL. Point mutation I634A in the glucocorticoid receptor causes embryonic lethality by reduced ligand binding. JOURNAL OF BIOLOGICAL CHEMISTRY 2022; 298(2):101574, 15 pages.
VAN DYK L, VERHOOG NJD, LOUW A. Combinatorial treatments of tamoxifen and SM6Met, an extract from Cyclopia subternata Vogel, are superior to either treatment alone in MCF-7 cells. Frontiers in Pharmacology 2022; 13:1017690, 17 pages.
YU Y, VAN DER ZWAAG M, WEDMAN JJ, PERMENTIER H, PLOMP N, JIA X, KANON B, EGGENS-MEIJER E, STRAUSS E, ET AL. Coenzyme A precursors flow from mother to zygote and from microbiome to host. MOLECULAR CELL 2022; 82:2650-2665.
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Chapters in Books
DU TOIT A, HOFMEYR JS, LOOS B. Measuring Autophagosome Flux. In: LOOS B, Wong E (eds.) Imaging and Quantifying Neuronal Autophagy, Springer, New York, USA, 2022: 67-78.
Doctorals completed
Click here for an up-to-date list.
JACKSON HC. An investigation into the role of adiposity and progestins used in menopausal hormone therapy in breast cancer. PhD, 2022. 200 pp. Promotor: AFRICANDER DJ. Medepromotor: Pheiffer C.
KOK M. Studies on the metabolism of N-substituted pantothenamides in blood-stage Plasmodium falciparum. PhD, 2022. 255 pp. Promotor: DE VILLIERS M. Medepromotor: STRAUSS E.
OLAYOKU FR. SM6Met, a Cyclopia subternata extract as a potential selective oestrogen receptor downregulator (SERD) for breast cancer. PhD, 2022. 386 pp. Promotor: LOUW A.?
Masters completed
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FRANTZ TC. The control of GAPDH on glycolytic flux in Lactococcus lactis and Plasmodium falciparum. MSc, 2022. 98 pp. Studieleier: VAN NIEKERK DD. Medestudieleier: SNOEP JL.
GIBSON H. An investigation into the circulating levels of 11-oxygenated androgens in South African women and their link to insulin resistance. MSc, 2022. 94 pp. Studieleier: STORBECK K. Medestudieleier: SNOEP JL.
MINNIE SL. An investigation into N-substituted pantoyltauramides as inhibitors of Streptococcus pneumoniae and Plasmodium falciparum. MSc, 2022. 109 pp. Studieleier: DE VILLIERS M. Medestudieleier: STRAUSS E.
MURRAY SM. An investigation into the effect of metabolism on the comparative androgenic activity of testosterone and 11-ketotestosterone. MSc, 2022. 90 pp. Studieleier: STORBECK K. Medestudieleier: AFRICANDER DJ.
PATEL VB. The influence of impaired CoA biosynthesis on prostate cancer cells. MSc, 2022. 120 pp. Studieleier: DE VILLIERS M. Medestudieleier: STORBECK K.
SHARP M. Investigating the effects of progestins used in contraception and menopausal hormone therapy on aromatase expression and activity. MSc, 2022. 86 pp. Studieleier: AFRICANDER DJ.
SIMONS M. Investigating the effects of progestins and adiposity on c-Jun N-terminal kinase (JNK) signalling in breast cancer. MSc, 2022. 90 pp. Studieleier: AFRICANDER DJ.
SITZER W. Investigation of protein complexes involved in coenzyme A biosynthesis. MSc, 2022. 72 pp. Studieleier: STRAUSS E. Medestudieleier: Woodward JD.
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Publications in 2021
Journal Articles (subsidised)
ABIDIN MZ, SARAVANAN T, STRAUSS E, POELARENDS GJ. The broad amine scope of pantothenate synthetase enables the synthesis of pharmaceutically relevant amides. Organic and Biomolecular Chemistry 2021; 19:4515-4519.
ALBERTS JF, DAVIDS I, MOLL W-D, SCHATZMAYR G, BURGER H-M, SHEPHARD GS, GELDERBLOM WCA. Enzymatic detoxification of the fumonisin mycotoxins during dry milling of maize. Food Control 2021; 123:107726, 9 pages.
APPANNA N, GIBSON H, GANGITANO E, DEMPSTER NJ, MORRIS K, GEORGE S, ARVANITI A, GATHERCOLE LL, KEEVIL B, PENNING TM, STORBECK K, TOMLINSON JW, NIKOLAOU N. Differential activity and expression of human 5β-reductase (AKR1D1) splice variants. JOURNAL OF MOLECULAR ENDOCRINOLOGY 2021; 66(3):181-194.
BARNARD L, DU TOIT T, SWART AC. Back where it belongs: 11β-hydroxyandrostenedione compels the re-assessment of C11-oxy androgens in steroidogenesis. Molecular and Cellular Endocrinology 2021; 525:111189, 17 pages.
BARNARD L, SCHIFFER L, LOUW-DU TOIT R, TAMBLYN JA, CHEN S, AFRICANDER DJ, ARLT W, FOSTER PA, STORBECK K. 11-Oxygenated Estrogens Are a Novel Class of Human Estrogens but Do not Contribute to the Circulating Estrogen Pool. Endocrinology 2021; 162(3):1-11.
BICK AJ, LOUW-DU TOIT R, SKOSANA SB, AFRICANDER DJ, HAPGOOD JP. Pharmacokinetics, metabolism and serum concentrations of progestins used in contraception. PHARMACOLOGY & THERAPEUTICS 2021; 222:107789, 18 pages.
BINATI RL, DU TOIT M, SNOEP JL, SALVETTI E, TORRIANI S. Transcriptional and Metabolic Response of Wine-Related Lactiplantibacillus plantarum to Different Conditions of Aeration and Nitrogen Availability. Fermentation 2021; 7:68, 16 pages.
BOOYSEN E, RAUTENBACH M, STANDER MA, DICKS LMT. Profiling the Production of Antimicrobial Secondary Metabolites by Xenorhabdus khoisanae J194 Under Different Culturing Conditions. Frontiers in Chemistry 2021; 9:article 626653, 15 pages.
CHAKAFANA G, MUDAU PT, ZININGA T, SHONHAI A. Characterisation of a unique linker segment of the Plasmodium falciparum cytosol localised Hsp110 chaperone. International Journal of Biological Macromolecules 2021; 180:272-285.
CHAKAFANA G, MUDAU PT, ZININGA T, SHONHAI A. Supporting data on characterisation of linker switch mutants of Plasmodium falciparum heat shock protein 110 and canonical Hsp70. Data in Brief 2021; 37:107177, 11 pages.
CHAKAFANA G, SPRACKLEN TF, KAMULI S, ZININGA T, SHONHAI A, NTUSI NAB, SLIWA K. Heat Shock Proteins: Potential Modulators and Candidate Biomarkers of Peripartum Cardiomyopathy. Frontiers in Cardiovascular Medicine 2021; 8:633013, 18 pages.
CLEMENTS TL, RAUTENBACH M, NDLOVU T, KHAN S, KHAN W. A Metabolomics and Molecular Networking Approach to Elucidate the Structures of Secondary Metabolites Produced by Serratia marcescens Strains. Frontiers in Chemistry 2021; 9:Article 633870, 16 pages.
DU TOIT T, SWART AC. Turning the spotlight on the C11-oxy androgens in human fetal development. Journal of Steroid Biochemistry and Molecular Biology 2021; 212:105946, 14 pages.
GRZEGORZEWSKI J, BRANDHORST J, GREEN KA, ELEFTHERLADOU D, DUPORT Y, BARTHORSCHT F, K?LLER A, YU JIA KE D, DE ANGELIS S, K?NIG M. PK-DB: pharmacokinetics database for individualized and stratified computational modeling. Nucleic Acids Research 2021; 49:D1358-D1364.
HARDY RS, BOTFIELD H, MARKEY K, MITCHELL JL, ALIMAJSTOROVIC Z, WESTGATE CSJ, SAGMEISTER M, FAIRCLOUGH RJ, OTTRIDGE RS, YIANGOU A, STORBECK K, ET AL. 11βHSD1 Inhibition with AZD4017 Improves Lipid Profiles and Lean Muscle Mass in Idiopathic Intracranial Hypertension. Journal of Clinical Endocrinology and Metabolism 2021; 106(1):174-187.
HOFMEYR JS. A biochemically-realisable relational model of the self-manufacturing cell. Biosystems 2021; 207:104463, 15 pages.
HORN S, SNOEP JL, VAN NIEKERK DD. Uncovering the efects of heterogeneity and parameter sensitivity on within?host dynamics of disease: malaria as a case study. BMC Bioinformatics 2021; 22:384, 21 pages.
KAYAMBA F, MALIMABE T, ADEMOLA IK, POOE OJ, KUSHWAHA ND, MAHLALELA M, VAN ZYL RL, GORDON M, MUDAU PT, ZININGA T, SHONHAI A, NYAMORI VO, KARPOORMATH R. Design and synthesis of quinoline-pyrimidine inspired hybrids as potential plasmodial inhibitors. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY 2021; 217:113330, 20 pages.
LILLY M, RHEEDER JP, PROCTOR RH, GELDERBLOM WCA. FUM gene expression and variation in fumonisin production of clonal isolates of Fusarium verticillioides MRC 826. World Mycotoxin Journal 2021; 14(2):121-137.
LOUW C, VAN SCHALKWYK FJ, CONRADIE R, LOUW RP, ENGELBRECHT Y, STORBECK K, SWART AC, VAN NIEKERK DD, SNOEP JL, SWART P. Computational modelling of the Δ4 and Δ5 adrenal steroidogenic pathways provides insight into hypocortisolism. Molecular and Cellular Endocrinology 2021; 526:111194, 8 pages.
MAGANGANA TP, MAKUNGA NP, LA GRANGE C, STANDER MA, FAWOLE OA, OPARA UL. Blanching Pre-Treatment Promotes High Yields, Bioactive Compounds, Antioxidants, Enzyme Inactivation and Antibacterial Activity of 'Wonderful' Pomegranate Peel Extracts at Three Different Harvest Maturities. Antioxidants 2021; 10(1119):1-25.
MAGANGANA TP, STANDER MA, MASONDO N, MAKUNGA NP. Steviol glycoside content and essential oil profiles of Stevia rebaudiana Bertoni in response to NaCl and polyethylene glycol as inducers of salinity and drought stress in vitro. Plant Cell, Tissue and Organ Culture 2021; 145:1-18.
MAKUMIRE S, DONGOLA TH, CHAKAFANA G, TSHIKONWANE L, CHAUKE CT, MAHARAJ T, ZININGA T, SHONHAI A. Mutation of GGMP Repeat Segments of Plasmodium falciparum Hsp70-1 Compromises Chaperone Function and Hop Co-Chaperone Binding. International Journal of Molecular Sciences 2021; 22:2226, 22 pages.
MASIKE K, STANDER MA, DE VILLIERS AJ. Recent applications of ion mobility spectrometry in natural product research. Journal of Pharmaceutical and Biomedical Analysis 2021; 195:113846: 8.
MASOUDI Y, VAN RENSBURG W, JENKINS B, RAUTENBACH M. The Influence of Cellulose-Type Formulants on Anti-Candida Activity of the Tyrocidines. Antibiotics 2021; 10(5):597, 16 pages.
MOJICA-BENAVIDES M, VAN NIEKERK DD, MIJALKOV M, SNOEP JL, MEHLIG B, VOLPE G, GOKS?R M, ADIELS CB. Intercellular communication induces glycolytic synchronization waves between individually oscillating cells . Proceedings of the National Academy of Sciences of the United States of America 2021; 118(6):e2010075118, 8 pages.
MOSTERT KJ, SHARMA N, VAN DER ZWAAG M, STAATS R, KOEKEMOER L, ANAND R, SIBON OCM, STRAUSS E. The Coenzyme A Level Modulator Hopantenate (HoPan) Inhibits Phosphopantotenoylcysteine Synthetase Activity. ACS Chemical Biology 2021; 16:2401-2414.
RAS TA, STRAUSS E, BOTES A. Evaluating the Genetic Capacity of Mycoplasmas for Coenzyme A Biosynthesis in a Search for New Anti-mycoplasma Targets. Frontiers in Microbiology 2021; 12:Article 791756, 18 pages.
RAUTENBACH M, KUMAR V, VOSLOO JA, MASOUDI Y, VAN WYK RJ, STANDER MA. Oligomerisation of tryptocidine C, a Trp-rich cyclodecapeptide from the antimicrobial tyrothricin complex. Biochimie 2021; 181:123-133.
ROHWER JM, SCHUTTE C, VAN DER SLUIS R. Functional Characterisation of Three Glycine N-Acyltransferase Variants and the Effect on Glycine Conjugation to Benzoyl–CoA. International Journal of Molecular Sciences 2021; 22:3129, 17 pages.
SAMODIEN S, DE KOCK M, JOUBERT E, SWANEVELDER S, GELDERBLOM WCA. Differential Cytotoxicity of Rooibos and Green Tea Extracts against Primary Rat Hepatocytes and Human Liver and Colon Cancer Cells ? Causal Role of Major Flavonoids. NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL 2021; 73(10):2050-2064.
SCHIFFER L, BOSSEY A, KEMPEGOWDA P, TAYLOR AE, AKERMAN I, SCHEEL-TOELLNER D, STORBECK K, ARLT W. Peripheral blood mononuclear cells preferentially activate 11-oxygenated androgens. European Journal of Endocrinology 2021; 184(3):357-367.
SIANGWATA S, WILLIAMS C, OMOSUN N, NGUBANE S, SMITH GS. Core- and peripheral-functionalised metallodendrimers for hydroformylation reactions: A review. Applied Catalysis A-General 2021; 626:118362, 12 pages.
SPIES LL, VERHOOG NJD, LOUW A. Acquired Glucocorticoid Resistance Due to Homologous Glucocorticoid Receptor Downregulation: A Modern Look at an Age-Old Problem. Cells 2021; 10:2529, 27 pages.
STOFBERG ML, CAILLET C, DE VILLIERS M, ZININGA T. Inhibitors of the Plasmodium falciparum Hsp90 towards Selective Antimalarial Drug Design: The Past, Present and Future. Cells 2021; 10:2849, 31 pages.
SWART AC, DU TOIT T, GOURGARI E, KIDD M, KEIL M, FAUCZ FR, STRATAKIS C. Steroid hormone analysis of adolescents and young women with polycystic ovarian syndrome and adrenocortical dysfunction using UPC2-MS/MS. PEDIATRIC RESEARCH 2021; 89:118-126.
UYS L, HOFMEYR JS, ROHWER JM. Coupling kinetic models and advection-diffusion equations. 1. Framework development and application to sucrose translocation and metabolism in sugarcane. in silico Plants 2021; 3(1):art. no. diab013, 18 pages.
UYS L, HOFMEYR JS, ROHWER JM. Coupling kinetic models and advection-diffusion equations. 2. Sensitivity analysis of an advection-diffusion-reaction model. in silico Plants 2021; 3(1):art. no. diab014, 14 pages.
VAN DER MERWE PDW, COTTERILL FPD, KANDZIORA M, WATTERS BR, NAGY B, GENADE T, FLUGEL T, SVENDSEN D, BELLSTEDT DU. Genomic fingerprints of palaeogeographic history: The tempo and mode of rift tectonics across tropical Africa has shaped the diversification of the killifish genus Nothobranchius (Teleostei: Cyprinodontiformes). Molecular Phylogenetics and Evolution 2021; 158:106988, 21 pages.
VAN NIEKERK DD, OOSTHUIZEN MM, DU TOIT F, SNOEP JL. Estimating merozoite release number and reinvasion efficiency in Plasmodium falciparum cell culture. Transactions of the Royal Society of South Africa 2021; 76(2):147-155.
VAN RENSBURG W, LAUBSCHER WE, RAUTENBACH M. High throughput method to determine the surface activity of antimicrobial polymeric materials. MethodsX 2021; 8:101593, 13 pages.
VAN SCHALKWYK FJ, STANDER MA, NSIZWANE M, MATHEE A, VAN WYK B-E. Fatal pyrrolizidine alkaloid poisoning of infants caused by adulterated Senecio coronatus. FORENSIC SCIENCE INTERNATIONAL 2021; 320:110680, 7 pages.
VERMEULEN RR, DEANE SM, DICKS LMT, ROHWER JM, VAN STADEN ADP. Manganese Privation-Induced Transcriptional Upregulation of the Class IIa Bacteriocin Plantaricin 423 in Lactobacillus plantarum Strain 423. Applied and Environmental Microbiology 2021; 87(21):e00976-21, 15 pages.
YILDIZ M, ISIK E, ABALI ZY, KESKIN M, OZBEK MN, BAS F, UCAKTURK SA, BUYUKINAN M, ONAL H, KARA C, STORBECK K, ET AL. Clinical and Hormonal Profiles Correlate With Molecular Characteristics in Patients With 11β-Hydroxylase Deficiency. Journal of Clinical Endocrinology and Metabolism 2021; 106(9):e3714-e3724.
Journal Articles (non-subsidised)
WEBER A, BELLSTEDT DU, M?LLER M. Die Gesneriaceen Afrikas und ihre vogelblütigen Vertreter: Streptocarpus dunnii und S. myoporoides. Der Palmengarten 2021; 85:124-137.
Chapters in Books
ZININGA T, B?TTGER E, MULTHOFF G. Role of Heat Shock Proteins in Immune Modulation in Malaria. In: Shonhai A, Picard D, Blatch GL (eds.) Heat Shock Proteins of Malaria, Advances in Experimental Medicine and Biology, Springer Nature, Switzerland, 2021: 169-186.?
Doctorals completed
Click here for an up-to-date list.
?CARTWRIGHT M. Progestins and breast cancer: Significance of progesterone receptor isoforms and their altered ratios. PhD, 2021. 323 pp. Promotor: AFRICANDER DJ. Medepromotor: LOUW-DU TOIT R.
K?HN S. Quantifying Insulin Signaling in Mouse C2C12 Skeletal Muscle: A Minimal Modelling Approach. PhD, 2021. 233 pp. Promotor: SNOEP JL, Westerhoff HW.
MASIKE K. The development of standardized ion mobility and mass spectrometry metabolomics methods for the characterization of plant phenolics. PhD, 2021. 211 pp. Promotor: STANDER MA. Medepromotor: DE VILLIERS AJ.
MOSTERT KJ. In vitro reconstitution of the coenzyme A biosynthesis pathway for the study of its regulation and disruption by possible inhibitors . PhD, 2021. 184 pp. Promotor: STRAUSS E. Medepromotor: SNOEP JL.?
Masters completed
Click here for an up-to-date list.??
BOTHMA K. Antibody production against Staphylococcus aureus CoA biosynthesis enzymes and their application in protein level quantification. MSc Biochemie, 2021. 122 pp. Studieleier: DE VILLIERS M. Medestudieleier: BELLSTEDT DU.
CONRADIE LT. A bioinformatics tool to detect physical gene clusters in functional genomics data. MSc Biochemie, 2021. 111 pp. Studieleier: PATTERTON H.
DALE L. Examining plasminogen activator-inhibitor-1 and C-reactive protein regulation by glucocorticoids and pro-inflammatory cytokines in liver cell lines. MSc Biochemie, 2021. 178 pp. Studieleier: VERHOOG NJD.
DE VILLIERS WH. Mathematical modelling of glycolysis in differentiated mouse adipocytes 3T3-L1. MSc Biochemie, 2021. 99 pp. Studieleier: SNOEP JL. Medestudieleier: KOURIL T, VAN NIEKERK DD, VAN DYK JB.
FISHER JC. Modular control analysis and its application to glucose metabolism in Plasmodium falciparum-infected erythrocytes. MSc Biochemie, 2021. 119 pp. Studieleier: SNOEP JL. Medestudieleier: VAN NIEKERK DD.
GREEN NJ. Co-regulation of insulin signalling by glucocorticoids and pro-inflammatory cytokines. MSc Biochemie, 2021. 117 pp. Studieleier: VERHOOG NJD. Medestudieleier: LOUW A.
KELLOW-WEBB SM. The Expression, Purification and Characterization of Escherichia coli NudL, a Putative Coenzyme A Hydrolase. MSc, 2021. 87 pp. Studieleier: STRAUSS E. Medestudieleier: DE VILLIERS M.
KOTZE S. The role of a positive feedforward loop in regulating yeast glycolytic intermediate dynamics . MSc Biochemie, 2021. 86 pp. Studieleier: SNOEP JL. Medestudieleier: VAN NIEKERK DD.
MASOUDI Y. Characterisation and formulation of natural cyclodecapeptides with anti-Candida activity. MSc Biochemie, 2021. 140 pp. Studieleier: RAUTENBACH M.
MYBURGH L. Modelling the insulin signaling pathway and glucose transport induction in differentiated 3T3-L1 mouse adipocytes. MSc Biochemie, 2021. 113 pp. Studieleier: SNOEP JL. Medestudieleier: VAN NIEKERK DD.
OCTOBER CA. Mathematical modelling of glycolysis in cancer cell lines. MSc Biochemie, 2021. 90 pp. Studieleier: SNOEP JL. Medestudieleier: KOURIL T, VAN NIEKERK DD.
RADEMEYER AZ. Investigating the influence of the progesterone receptor isoforms on androgen receptor activity in breast and prostate cancer cell lines. MSc Biochemie, 2021. 99 pp. Studieleier: AFRICANDER DJ. Medestudieleier: STORBECK K.
RELLING E. An Investigation into the Progestogenic Activity of 11-Ketotestosterone. MSc Biochemie, 2021. 95 pp. Studieleier: STORBECK K. Medestudieleier: AFRICANDER DJ.
RIEKERT B. Quantitation of the effects of cellular glucocorticoid receptor concentration on dimerization. MSc Biochemie, 2021. 135 pp. Studieleier: LOUW A. Medestudieleier: ROHWER JM.
RUST FC. On the relationship between thermodynamic disequilibrium ratio and flux control of metabolic pathways. MSc Biochemie, 2021. 86 pp. Studieleier: SNOEP JL. Medestudieleier: VAN NIEKERK DD.
SPIES LL. The effect of GRα dimerization state on receptor turnover. MSc Biochemie, 2021. 145 pp. Studieleier: LOUW A. Medestudieleier: VERHOOG NJD.
VAN DER BERG A. Investigating the effects of treatment in HIV disease models. MSc Biochemie, 2021. 102 pp. Studieleier: VAN NIEKERK DD. Medestudieleier: SNOEP JL.
VISSER A. Examining the transcription factor FOXO1 and its regulation by glucocorticoids and inflammatory mediators in hepatic insulin signalling. MSc Biochemie, 2021. 154 pp. Studieleier: VERHOOG NJD.
WELLS FB. Investigating the developmental changes in the gut microbiome of naturally reared ostrich chicks. MSc Biochemie, 2021. 166 pp. Studieleier: BOTES A. Medestudieleier: BELLSTEDT DU.??
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Publications in 2020
Journal Articles (subsidised)
AFRICANDER DJ, LOUW-DU TOIT R. Progestins in menopausal hormone therapy and breast cancer risk: The debate continues. Current Opinion in Endocrine and Metabolic Research 2020; 15:24-30.
BARNARD L, NIKOLAOU N, LOUW C, SCHIFFER L, GIBSON H, GILLIGAN L, GANGITANO E, SNOEP JL, ARLT W, TOMLINSON JW, STORBECK K. The A-ring reduction of 11-ketotestosterone is efficiently catalysed by AKR1D1 and SRD5A2 but not SRD5A1. Journal of Steroid Biochemistry and Molecular Biology 2020; 202:art no. 105724, 10 pages.
BARNARD M, MOSTAGHEL E, AUCHUS RJ, STORBECK K. The role of adrenal derived androgens in castration resistant prostate cancer. Journal of Steroid Biochemistry and Molecular Biology 2020; 197:art no. 105506, 14 pages.
BELLSTEDT DU. The building blocks and origins of life. HTS Theological Studies/ Teologiese Studies 2020; 76(1):a6054, 7 pages.
BUTMAN HS, KOTZ? TJ, DOWD CS, STRAUSS E. Vitamin in the crosshairs: Targeting Pantothenate and Coenzyme A biosynthesis for new antituberculosis agents. Frontiers in Cellular and Infection Microbiology 2020; 10:art no. 605662, 33 pages.
DU TOIT T, SWART AC. The 11β-hydroxyandrostenedione pathway and C11-oxy C21 backdoor pathway are active in benign prostatic hyperplasia yielding 11ketotestosterone and 11keto-progesterone. Journal of Steroid Biochemistry and Molecular Biology 2020; 196:art no. 105497, 14 pages.
DU TOIT T, VAN ROOYEN D, STANDER MA, ATKIN SL, SWART AC. Analysis of 52 C19 and C21 steroids by UPC2-MS/MS: Characterising the C11-oxy steroid metabolome in serum. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 2020; 1152:art no. 122243, 20 pages.
ENFIELD K, CARTWRIGHT M, LOUW-DU TOIT R, AVENANT C, AFRICANDER DJ, HAPGOOD JP. Characterisation of progestins used in hormonal contraception and progesterone via the progesterone receptor. Biochemical and Biophysical Research Communications 2020; 533:879-885.
GURAN T, KARA C, YILDIZ M, BITKIN EC, HAKLAR G, LIN J-C, KESKIN M, BARNARD L, ANIK A, CATLI G, STORBECK K, ET AL ET AL. Revisiting Classical 3β-hydroxysteroid Dehydrogenase 2 Deficiency: Lessons from 31 Pediatric Cases. Journal of Clinical Endocrinology and Metabolism 2020; 105(4):e1718-e1728.
HOFMEYR JS. Kinetic modelling of compartmentalised reaction networks. BIOSYSTEMS 2020; 197:art no. 104203, 17 pages.
JOKONYA S, LANGLAIS MAP, LESHABANE M, READER PW, VOSLOO JA, PFUKWA R, COERTZEN D, BIRKHOLTZ L, RAUTENBACH M, KLUMPERMAN L. Poly(N-vinylpyrrolidone) antimalaria conjugates of membrane-disruptive peptides. Biomacromolecules 2020; 21(12):5053-5066.
LEBEPE CM, MATAMBANADZO PR, MAKHOBA XH, ACHILONU I, ZININGA T, SHONHAI A. Comparative Characterization of Plasmodium falciparum Hsp70-1 Relative to E. coli DnaK Reveals the Functional Specificity of the Parasite Chaperone. Biomolecules 2020; 10:art no. 856, 21 pages.
LI N, OAKES JA, STORBECK K, CUNLIFFE VT, KRONE NP. The P450 side-chain cleavage enzyme Cyp11a2 facilitates steroidogenesis in zebrafish. JOURNAL OF ENDOCRINOLOGY 2020; 244(2):309-321.
LOOS B, KLIONSKY DJ, DU TOIT A, HOFMEYR JS. On the relevance of precision autophagy flux control in vivo - Points of departure for clinical translation. Autophagy 2020; 16(4):750-762.
LOUW-DU TOIT R, HAPGOOD JP, AFRICANDER DJ. A direct comparison of the transcriptional activities of progestins used in contraception and menopausal hormone therapy via the mineralocorticoid receptor. Biochemical and Biophysical Research Communications 2020; 526:466-471.
L?BCKER N, BLOEM LM, DU TOIT T, SWART P, DE BRUYN PJN, SWART AC, MILLAR RP. What's in a whisker? High-throughput analysis of twenty-eight C19 and C21 steroids in mammalian whiskers by ultra-performance convergence chromatography-tandem mass spectrometry. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 2020; 1141:art no. 122028, 10 pages.
MAKUMIRE S, ZININGA T, VAHOKOSKI J, KURSULA I, SHONHAI A. Biophysical analysis of Plasmodium falciparum Hsp70-Hsp90 organising protein (PfHop) reveals a monomer that is characterised by folded segments connected by flexible linkers. PLoS One 2020; 15(4):e0226657, 13 pages.
MARCHI D, SANTHAKUMAR K, MARKHAM E, LI N, STORBECK K, KRONE NP, CUNLIFFE VT, VAN EEDEN FJM. Bidirectional crosstalk between Hypoxia-Inducible Factor and glucocorticoid signaling in zebrafish larvae. PLoS Genetics 2020; 16(5):e1008757, 28 pages.
MASIKE K, DE VILLIERS AJ, HOFFMAN EW, BRAND DJ, CAUSON T, STANDER MA. Detailed phenolic characterization of Protea pure and hybrid cultivars by liquid chromatography-ion mobility-high resolution mass spectrometry (LC-IM-HR-MS). Journal of Agricultural and Food Chemistry 2020; 68(2):485-502.
MASIKE K, DE VILLIERS AJ, HOFFMAN EW, STANDER MA. Application of metabolomics tools to determine possible biomarker metabolites linked to leaf blackening in Protea. Journal of Agricultural and Food Chemistry 2020; 68(45):12595-12605.
NAGHILOO S, BELLSTEDT DU, CLASSEN-BOCKHOFF R. Pollination biology in Roepera (Zygophyllaceae): How flower structure and shape influence foraging activity. PLANT SPECIES BIOLOGY 2020; 35:72-80.
NAGY B, WATTERS BR, VAN DER MERWE PDW, COTTERILL FPD, BELLSTEDT DU. Review of the Nothobranchius ugandensis species group from the inland plateau of eastern Africa with descriptions of six new species (Teleostei: Nothobranchiidae). ICHTHYOLOGICAL EXPLORATION OF FRESHWATERS 2020; 30(1):21-73.
NEVELING D, AHIRE J, LAUBSCHER WE, RAUTENBACH M, DICKS LMT. Correction to: Genetic and Phenotypic Characteristics of a Multi strain Probiotic for Broilers. Current Microbiology 2020; 77:1955-1956.
NEVELING D, AHIRE J, LAUBSCHER WE, RAUTENBACH M, DICKS LMT. Genetic and Phenotypic Characteristics of a Multi strain Probiotic for Broilers. Current Microbiology 2020; 77:369-387.
NGUGI G, LE P?CHON T, MARTOS F, PAILLER T, BELLSTEDT DU, BYTEBIER B. Phylogenetic relationships amongst the African genera of subtribe Orchidinae s.l. (Orchidaceae; Orchideae): Implications for subtribal and generic delimitations. Molecular Phylogenetics and Evolution 2020; 153:art no. 106946, 8 pages.
OAKES JA, BARNARD L, STORBECK K, CUNLIFFE VT, KRONE NP. 11β-Hydroxylase loss disrupts steroidogenesis and reproductive function in zebrafish. JOURNAL OF ENDOCRINOLOGY 2020; 247(2):197-212.
PADAYACHEE L, ROHWER JM, PILLAY CS. The thioredoxin redox potential and redox charge are surrogate measures for flux in the thioredoxin system. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 2020; 680:art no. 108231, 12 pages.
PERRECA E, ROHWER JM, GONZ?LEZ-CABANELAS D, LORETO F, SCHMIDT A, GERSHENZON J, WRIGHT LP. Effect of drought on the Methylerythritol 4-Phosphate (MEP) pathway in the Isoprene emitting conifer Picea glauca. Frontiers in Plant Science 2020; 11:art no. 546295, 14 pages.
SAASA V, LEMMER Y, MALWELA T, AKANDE A, BEUKES M, MWAKIKUNGA B. Effect of varying ethanol and water compositions on the acetone sensing properties of WO3 for application in diabetes mellitus monitoring. Materials Research Express 2020; 7:art no. 035905, 13 pages.
SAASA V, MALWELA T, LEMMER Y, BEUKES M, MWAKIKUNGA B. The hierarchical nanostructured Co-doped WO3/carbon and their improved acetone sensing performance. MATERIALS SCIENCE IN SEMICONDUCTOR PROCESSING 2020; 117:art no. 105157, 9 pages.
SHARMA N, AHALAWAT N, SANDHU P, STRAUSS E, MONDAL J, ANAND R. Role of allosteric switches and adaptor domains in long-distance cross-talk and transient tunnel formation. Science Advances 2020; 6:eaay7919, 11 pages.
SHEN L, KOHLHAAS M, ENOKI J, MEIER R, SCH?NENBERGER B, WOHLGEMUTH R, KOURIST R, NIEMEYER F, VAN NIEKERK DD, BR?SEN C, NIEMEYER J, SNOEP JL, SIEBERS B. A combined experimental and modelling approach for the Weimberg pathway optimisation. Nature Communications 2020; 11:art no. 1098, 13 pages.
SPERGSER J, BOTES A, NEL TI, RUPPITSCH W, LEPUSCHITZ S, LANGER S, RIES S, DINHOPL N, SZOSTAK M, LONCARIC I, BUSSE H-J. Mycoplasma nasistruthionis sp. nov. and Mycoplasma struthionis sp. nov. isolated from ostriches with respiratory disease. SYSTEMATIC AND APPLIED MICROBIOLOGY 2020; 43:art no. 126047, 7 pages.
SPRY C, BARNARD L, KOK M, POWELL AK, MAHESH D, TJHIN ET, SALIBA KJ, STRAUSS E, DE VILLIERS M. Toward a Stable and Potent Coenzyme A Targeting Antiplasmodial Agent: Structure?Activity Relationship Studies of N Phenethyl-α-methyl-pantothenamide. ACS Infectious Diseases 2020; 6:1844-1854.
STORBECK K, MCNAMARA KM. The importance of mass spectrometry in unravelling steroid action in breast cancer. Current Opinion in Endocrine and Metabolic Research 2020; 15:57-62.
SWART AC, DU TOIT T, GOURGARI E, KIDD M, KEIL M, FAUCZ FR, STRATAKIS C. Steroid hormone analysis of adolescents and young women with polycystic ovarian syndrome and adrenocortical dysfunction using UPC?-MS/MS. PEDIATRIC RESEARCH 2020; doi.org/10.1038/s41390-020-0870-1:0.
TSHALIBE RS, RHEEDER JP, ALBERTS JF, TALJAARD-KRUGELL C, GELDERBLOM WCA, SHEPHARD GS, LOMBARD MJ, BURGER H-M. Multi-mycotoxin exposure of children (0-24 months) in rural maize-subsistence farming areas of the Eastern Cape Province, South Africa. World Mycotoxin Journal 2020; 13(3):401-410.
VAN ROOYEN D, YADAV R, SCOTT EE, SWART AC. CYP17A1 exhibits 17αhydroxylase/17,20-lyase activity towards 11β-hydroxyprogesterone and 11-ketoprogesterone metabolites in the C11-oxy backdoor pathway. Journal of Steroid Biochemistry and Molecular Biology 2020; 199:art no. 105614, 12 pages.
VIVIERS CGA, DU TOIT A, PEROLD WJ, LOOS B, HOFMEYR JH. A resistive biosensor for the detection of LC3 Protein in Autophagy. IEEE SENSORS JOURNAL 2020; 20(10):5119-5129.
VOSLOO JA, RAUTENBACH M. Following tyrothricin peptide production by Brevibacillus parabrevis with electrospray mass spectrometry. BIOCHIMIE 2020; 179:101-112.
WATTERS BR, NAGY B, VAN DER MERWE PDW, COTTERILL FPD, BELLSTEDT DU. Redescription of the seasonal killifish species Nothobranchius ocellatus and description of a related new species Nothobranchius matanduensis, from eastern Tanzania (Teleostei: Nothobranchiidae). ICHTHYOLOGICAL EXPLORATION OF FRESHWATERS 2020; 30(2):151-178.
WATTERS BR, NAGY B, VAN DER MERWE PDW, COTTERILL FPD, BELLSTEDT DU. Review of the Nothobranchius taeniopygus species group from central and western Tanzania with descriptions of five new species and redescription of Nothobranchius taeniopygus (Teleostei: Nothobranchiidae). ICHTHYOLOGICAL EXPLORATION OF FRESHWATERS 2020; 29(3):239-278.
Journal Articles (non-subsidised)
DEPFENHART M, LEMPERLE G, MEYER M, RAUTENBACH M, BERTOSSI D, DE VILLIERS D. A SARS-CoV-2 Prophylactic and Treatment; A Counter Argument Against The Sole Use of Chloroquine. American Journal of Biomedical Science & Research 2020; 8(4):248-251.Chapters in Books
DU TOIT A, LOOS B, HOFMEYR JS. Supply and demand analysis of Autophagy. In: Nagrath D (ed.) Metabolic Flux Analysis in Eukaryotic Cells, Springer Science & Business Media, New York, USA, 2020: 345-358.
Patents
KLUMPERMAN L, READER PW, RAUTENBACH M. Conjugate for treating malaria. Patent No. US 10,479,854, USA 2019.
RAUTENBACH M, VAN RENSBURG W. Method for preventing or treating microbial growth on a manufactured product. Patent No. AU2015270120, Australia 2019.
RAUTENBACH M, VAN RENSBURG W. Method for preventing or treating microbial growth on a manufactured product. Patent No. US 10,512,271, USA 2019.??
Doctorals completed
BARNARD L. An investigation into the biosynthesis, metabolism and activity of 11-Oxygenated estrogens. PhD, 2020. 175 pp. Promotor: STORBECK K. Medepromotor: Arlt W.
BARNARD M. An investigation into the role of 11-oxygenated androgens in castration resistant prostate cancer. PhD, 2020. 217 pp. Promotor: STORBECK K. Medepromotor: AFRICANDER DJ.
LOUW C. Computational modelling of steroid hormone biosynthesis and metabolism. PhD, 2020. 152 pp. Promotor: SNOEP JL. Medepromotor: VAN NIEKERK DD.
MAREE JP. Identification and genomic distribution of select combinatorial post-translational modification patterns of Trypanosoma brucei histones. PhD, 2020. 166 pp. Promotor: PATTERTON H. Medepromotor: LOUW A.
MOGWERA KSP. Membrane permeability and transport studies of Coenzyme A, its precursors and antimetabolites. PhD, 2020. 117 pp. Promotor: STRAUSS E. Medepromotor: DE VILLIERS M.
VAN DYK JB. Mathematical modelling of glycolysis in skeletal muscle cells. PhD, 2020. 168 pp. Promotor: SNOEP JL. Medepromotor: VAN NIEKERK DD.
VAN RENSBURG W. The tyrocidines in the creation of antimicrobial cellulose and sterilising materials. PhD, 2020. 203 pp. Promotor: RAUTENBACH M.
VAN ROOYEN D. An investigation into the biosynthesis and metabolism of adrenal C11-oxy steroids. PhD, 2020. 287 pp. Promotor: SWART AC. Medepromotor: VERHOOG NJD.
Masters completed
?BADENHORST M. Investigating the dynamics of hydrogen peroxide metabolism in the peroxiredoxin system of Saccharomyces cerevisiae. MSc Biochemie, 2020. 103 pp. Studieleier: ROHWER JM. Medestudieleier: Pillay CS.
BURGER A. An in vitro investigation into the effects of 11-oxygenated androgens in muscle. MSc Biochemie, 2020. 66 pp. Studieleier: STORBECK K. Medestudieleier: Kohn T, SNOEP JL.
HEITMANN S. Characterising the gut microbiome of ostrich chicks reared under intensive conditions. MSc Biochemie, 2020. 188 pp. Studieleier: BOTES A.
MEYER JP. Whole-body modelling of glucose and lactate dynamics in Plasmodium falciparum malaria. MSc Biochemie, 2020. 166 pp. Studieleier: VAN NIEKERK DD. Medestudieleier: SNOEP JL.
OCTOBER FM. Effect of yeasts and oenological parameters on acetaldehyde production during alcoholic fermentation of South African grape must. MSc Biochemie, 2020. 59 pp. Studieleier: Van Jaarsveld FP. Medestudieleier: DU TOIT WJ, ROHWER JM.
SHAW K. Quantification of the insulin response in rat L6 skeletal muscle cells. MSc Biochemie, 2020. 59 pp. Studieleier: SNOEP JL. Medestudieleier: VAN NIEKERK DD.
SPEELMAN TS. The effect of acute phase proteins on hepatic insulin signalling. MSc Biochemie, 2020. 133 pp. Studieleier: VERHOOG NJD. Medestudieleier: LOUW A.
WISSING J. An investigation into the effects of macromolecular crowding on the kinetics of upper glycolytic enzymes in Saccharomyces cerevisiae. MSc Biochemie, 2020. 55 pp. Studieleier: ROHWER JM.??
Publications in 2019
Journal Articles (subsidised)
ABRAHAMS S, SAMODIEN S, LILLY M, JOUBERT E, GELDERBLOM WCA. Differential Modulation of Gene Expression Encoding Hepatic and Renal Xenobiotic Metabolizing Enzymes by an Aspalathin-Enriched Rooibos Extract and Aspalathin. PLANTA MEDICA 2019; 85:6-13.
AFZAN A, BR?ANT L, BELLSTEDT DU, GRANT JR, QUEIROZ EF, WOLFENDER J-L, KISSLING J. Can biochemical phenotype, obtained from herbarium samples, help taxonomic decisions? – A case study using Gentianaceae. TAXON 2019; 68(4):771-782.
ALBERTS JF, SCHATZMAYR G, MOLL W-D, DAVIDS I, RHEEDER JP, BURGER H-M, SHEPHARD GS, GELDERBLOM WCA. Detoxification of the Fumonisin Mycotoxins in Maize: An Enzymatic Approach. Toxins 2019; 11:art no. 523, 15 pages.
BADENHORST M, BARRY CJ, SWANEPOEL CJ, VAN STADEN CT, WISSING J, ROHWER JM. Workflow for Data Analysis in Experimental and Computational Systems Biology: Using Python as 'Glue'. Processes 2019; 7:art. no. 460, 17 pages.
CLOETE WJ, HAYWARD S, SWART P, KLUMPERMAN L. Degradation of proteins and starch by combined immobilization of protease, alpha-amylase and beta-galactosidase on a single electrospun nanofibrous membrane. Molecules 2019; 24(3):508: 9.
DE JAGER C, ANECK-HAHN N, VAN ZIJL MC, HAYWARD S, SWART P, GENTHE B. Endocrine disrupting chemicals in commercially available cling film brands in South Africa. Human and Ecological Risk Assessment (HERA) 2019; 25(6):1633-1644.
DOMINGO RW, VAN DER WESTHUYZEN R, HAMANN AR, MOSTERT KJ, BARNARD L, PAQUET T, TJHIN ET, SALIBA KJ, VAN OTTERLO WAL, STRAUSS E. Overcoming synthetic challenges in targeting coenzyme A biosynthesis with the antimicrobial natural product CJ-15,801. MedChemComm 2019; 10:2118-2125.
DREYER J, RAUTENBACH M, BOOYSEN E, VAN STADEN ADP, DEANE SM, DICKS LMT. Xenorhabdus khoisanae SB10 produces Lys-rich PAX lipopeptides and a Xenocoumacin in its antimicrobial complex. BMC MICROBIOLOGY 2019; 19(1):art no. 132, 11 pages.
GENT R, DU TOIT T, BLOEM LM, SWART AC. The 11β-hydroxysteroid dehydrogenase isoforms: pivotal catalytic activities yield potent C11-oxy C19 steroids with 11βHSD2 favouring 11-ketotestosterone, 11-ketoandrostenedione and 11-ketoprogesterone biosynthesis. Journal of Steroid Biochemistry and Molecular Biology 2019; 189:116-126.
GENT R, DU TOIT T, SWART AC. 11α-Hydroxyprogesterone, a potent 11β-hydroxysteroid dehydrogenase inhibitor, is metabolised by steroid-5α-reductase and cytochrome P450 17α-hydroxylase/17,20-lyase to produce C11α-derivatives of 21-deoxycortisol and 11-hydroxyandrostenedione in vitro. Journal of Steroid Biochemistry and Molecular Biology 2019; 191:105369. 11 pages.
GUSTAVSSON A-K, BANAEIYAN AA, VAN NIEKERK DD, SNOEP JL, ADIELS CB, GOKS?R M. Studying Glycolytic Oscillations in Individual Yeast Cells by Combining Fluorescence Microscopy with Microfluidics and Optical Tweezers. Current Protocols in Cell Biology 2019; 82(1):e70, 26 pages.
HAFERKAMP P, TJADEN B, SHEN L, BR?SEN C, KOURIL T, SIEBERS B. The Carbon Switch at the Level of Pyruvate and Phosphoenolpyruvate in Sulfolobus solfataricus P2. Frontiers in Microbiology 2019; 10:art. no. 757, 13 pages.
JOUBERT E, DE BEER D, MALHERBE CJ, MULLER M, LOUW A, GELDERBLOM WCA. Formal honeybush tea industry reaches 20 year milestone- progress of product research targeting phenolic composition, quality and bioactivity. South African Journal of Botany 2019; 127:58-79.
JUHL DW, VAN RENSBURG W, BOSSIS X, VOSLOO JA, RAUTENBACH M, BECHINGER B. Tyrocidine A interactions with saccharides investigated by CD and NMR spectroscopies. JOURNAL OF PEPTIDE SCIENCE 2019; 25(5):e3163, 11 Pages.
LE MAITRE NC, PIRIE MD, BELLSTEDT DU. An approach to determining anthocyanin synthesis enzyme gene expression in an evolutionary context: an example from Erica plukenetii. Annals of Botany 2019; 124:121-129.
LE MAITRE NC, PIRIE MD, BELLSTEDT DU. Floral Color, Anthocyanin Synthesis Gene Expression and Control in Cape Erica Species. Frontiers in Plant Science 2019; 10:e1565, 9 pages.
LOOS B, KLIONSKY DJ, DU TOIT A, HOFMEYR JS. On the relevance of precision autophagy flux control in vivo - Points of departure for clinical translation. Autophagy 2019; 2019:13 pages.
LOUW A. GR Dimerization and the Impact of GR Dimerization on GR Protein Stability and Half-Life. Frontiers in Immunology 2019; 10:e1693, 15 pages.
NAGHILOO S, BELLSTEDT DU, CLABEN-BOCKHOFF R. The plasticity of breeding system in arid-adapted Zygophylloideae. JOURNAL OF ARID ENVIRONMENTS 2019; 162:1-9.
NEAL ML, K?NIG M, NICKERSON D, MISIRLI G, KALBASI R, DR?GER A, ATALAG K, CHELLIAH V, COOLING MT, COOK DL, CROOK S, DE ALBA M, FRIEDMAN SH, GARNY A, GENNARI H, GLEESON P, GOLEBIEWSKI M, HUCKA M, JUTY N, MYERS C, OLIVIER BG, SAURO HM, SCHARM M, SNOEP JL, ET. Harmonizing semantic annotations for computational models in biology. BRIEFINGS IN BIOINFORMATICS 2019; 20(2):540-550.
OAKES JA, LI N, WISTOW BRC, GRIFFIN A, BARNARD L, STORBECK K, CUNLIFFE VT, KRONE NP. Ferredoxin 1b Deficiency Leads to Testis Disorganization, Impaired Spermatogenesis, and Feminization in Zebrafish. Endocrinology 2019; 160(10):2401-2416.
PERKINS MS, LOUW-DU TOIT R, AFRICANDER DJ. Hormone therapy and breast cancer: emerging steroid receptor mechanisms. JOURNAL OF MOLECULAR ENDOCRINOLOGY 2019; 61(4):R133-R160.
PIRIE MD, KANDZIORA M, N?RK NM, LE MAITRE NC, MUGRABI DE KUPPLER AL, GEHRKE B, OLIVER EGH, BELLSTEDT DU. Leaps and bounds: geographical and ecological distance constrained the colonisation of the Afrotemperate by Erica. BMC Evolutionary Biology 2019; 19:art. no. 222, 12 pages.
R?GNIER M, POLIZZI A, LUKOWICZ C, SMATI S, LASSERRE F, LIPPI Y, NAYLIES C, LAFFITTE J, B?TOULI?RES C, MONTAGNER A, DUCHEIX S, GOURBEYRE P, ELLERO-SIMATOS S, GELDERBLOM WCA, ET AL. The protective role of liver X receptor (LXR) during fumonisin B1-induced hepatotoxicity. ARCHIVES OF TOXICOLOGY 2019; 93:505-517.
SAASA V, BEUKES M, LEMMER Y, MWAKIKUNGA B. Blood Ketone Bodies and Breath Acetone Analysis and their Correlations in Type 2 Diabetes Mellitus. Diagnostics 2019; 9(4):art no: 224, 10 pages.
SCHIFFER L, BARNARD L, BARANOWSKI ES, GILLIGAN L, TAYLOR AE, ARLT W, SHACKLETON CHL, STORBECK K. Human steroid biosynthesis, metabolism and excretion are differentially reflected by serum and urine steroid metabolomes: A comprehensive review. Journal of Steroid Biochemistry and Molecular Biology 2019; 194:art. no. 105439, 25 pages.
SHEPHARD GS, BURGER H-M, RHEEDER JP, ALBERTS JF, GELDERBLOM WCA. The effectiveness of regulatory maximum levels for fumonisin mycotoxins in commercial and subsistence maize crops in South Africa. Food Control 2019; 97:77-80.
SKOSANA SB, WOODLAND JG, CARTWRIGHT M, ENFIELD K, KOMANE M, LOUW-DU TOIT R, VAN DER SPUY ZM, AVENANT C, AFRICANDER DJ, STORBECK K, HAPGOOD JP. Differential metabolism of clinically-relevant progestogens in cell lines and tissue: Implications for biological mechanisms. Journal of Steroid Biochemistry and Molecular Biology 2019; 189:145-153.
STANDER MA, REDELINGHUYS H, MASIKE K, LONG H, VAN WYK B-E. Patterns of Variation and Chemosystematic Significance of Phenolic Compounds in the Genus Cyclopia (Fabaceae, Podalyrieae). Molecules 2019; 24:2352, 20 pages.
STEYN HM, VON STADEN L, BELLSTEDT DU, VAN DER MERWE PDW, VAN WYK AE. Notes on the phytogeography and conservation status of the genus Acanthopsis (Acantheae, Acanthaceae). Phytotaxa 2019; 415(4):157-178.
STORBECK K, SCHIFFER L, BARANOWSKI ES, CHORTIS V, PRETE A, BARNARD L, GILLIGAN L, TAYLOR AE, IDKOWIAK J, ARLT W, SHACKLETON CHL. Steroid Metabolome Analysis in Disorders of Adrenal Steroid Biosynthesis and Metabolism. ENDOCRINE REVIEWS 2019; 40(6):1605-1625.
SWART AC, JOHANNES ID, SATHYAPALAN T, ATKIN SL. The Effect of Soy Isoflavones on Steroid Metabolism. Frontiers in Endocrinology 2019; 10:art. no. 229, 10 Pages.
VAN NIEKERK DD, GUSTAVSSON A-K, MOJICA-BENAVIDES M, ADIELS CB, GOKS?R M, SNOEP JL. Phosphofructokinase controls the acetaldehyde-induced phase shift in isolated yeast glycolytic oscillators. BIOCHEMICAL JOURNAL 2019; 476:353-363.
VOLKE DC, ROHWER JM, FISCHER R, JENNEWEIN S. Investigation of the methylerythritol 4?phosphate pathway for microbial terpenoid production through metabolic control analysis. Microbial Cell Factories 2019; 18:e192, 15 pages.
VOSLOO JA, SNOEP JL, RAUTENBACH M. Modelling the variable incorporation of aromatic amino acids in the tyrocidines and analogous cyclodecapeptides. Journal of Applied Microbiology 2019; 127:1665-1676.
WIUM
M, JONKER HI, OLIVIER A, BELLSTEDT DU, BOTES A. DNA vaccines against
Mycoplasma elicit humoral immune responses in ostriches. Frontiers in Immunology 2019; 10:art. no. 1061, 9 pages.
Chapters in Books
STANFORD
NJ, SCHARM M, DOBSON PD, GOLEBIEWSKI M, HUCKA M, KOTHAMACHU VB,
NICKERSON D, OWEN S, PAHLE J, WITTIG U, WALTEMATH D, GOBLE C, MENDES P,
SNOEP JL. Data Management in Computational Systems Biology: Exploring
Standards, Tools, Databases, and Packaging Best Practices. In: Oliver
SG, Castilla JC (eds.) Yeast Systems Biology. Methods in Molecular Bilogy, vol 2049, Humana, New York, USA, 2019: 285-314.
???Doctorals completed
BALCOMB BH. The Staphylococcus aureus redoxome: Characterization of enzymes involved in oxidative stress resistance and survival. PhD, 2019. 214 pp. Promotor: STRAUSS E.
DU TOIT A. Measuring and Modelling Autophagic Flux. PhD, 2019. 232 pp. Promotor: LOOS B. Medepromotor: HOFMEYR JS.
JANA C. Synthesis, profiling and mode of action studies of PanSulfAms as inhibitors of coenzyme A biosynthesis and utilization. PhD, 2019. 182 pp. Promotor: STRAUSS E. Medepromotor: DE VILLIERS M.?
Masters completed
BRINK D. Investigating progestin-mediated crosstalk between the androgen receptor and estrogen receptor subtypes in breast cancer cell lines. MSc, 2019. 136 pp. Studieleier: AFRICANDER DJ.
EKSTEEN A. Investigating the role of inflammation, progestins and steroid receptors in breast cancer. MSc, 2019. 118 pp. Studieleier: AFRICANDER DJ. Medestudieleier: VERHOOG NJD.
HORN S. A Comparative Analysis of Within-host Models of Malaria Infection with Immune Response. MSc, 2019. 121 pp. Studieleier: VAN NIEKERK DD. Medestudieleier: SNOEP JL.
MAMHENDE PGM. Investigation of the antifungal activity of tryptophan-rich cyclic peptides. MSc, 2019. 162 pp. Studieleier: RAUTENBACH M.
MUIYSER SM. The role of PFK in the oscillatory yeast strain x2180. MSc, 2019. 93 pp. Studieleier: VAN NIEKERK DD. Medestudieleier: SNOEP JL.
ODENDAAL JCW. Glycolytic Flux Control of Glyceraldehyde 3-Phosphate Dehydrogenase in Yeast. MSc, 2019. 154 pp. Studieleier: SNOEP JL. Medestudieleier: VAN NIEKERK DD.
VAN WYK M. The eect of adenylate kinase on the glycolytic oscillations of Saccharomyces cerevisiae. MSc, 2019. 86 pp. Studieleier: SNOEP JL. Medestudieleier: VAN NIEKERK DD, KOURIL T.
VAN WYK RJ. Development of cyclodecapeptides from the tyrothricin complex as anticancer peptides. MSc, 2019. 174 pp. Studieleier: RAUTENBACH M.
Publications in 2018
Journal Articles (subsidised)
ABIDIN MZ, SARAVANAN T, ZHANG J, TEPPER PG, STRAUSS E, POELARENDS GJ. Modular enzymatic cascade synthesis of vitamin B5and its derivatives. CHEMISTRY-A EUROPEAN JOURNAL 2018; 24:17434-17438.
AFRICANDER DJ, STORBECK K. Steroid metabolism in breast cancer: Where are we and what are we missing?. Molecular and Cellular Endocrinology 2018; 466:86-97.
ANECK-HAHN NH, VAN ZIJL MC, SWART P, TRUEBODY BE, GENTHE B, CHARMIER J, DE JAGER C. Estrogenic activity, selected plasticizers and potential health risks associated with bottled water in South Africa. JOURNAL OF WATER AND HEALTH 2018; 16(2):253-262.
BARNARD L, MOSTERT KJ, VAN OTTERLO WAL, STRAUSS E. Developing pantetheinase-resistant pantothenamide antibacterials: Structural modification impacts on PanK interaction and mode of action. ACS Infectious Diseases 2018; 4(5):736-743.
BARNARD M, QUANSON J, MOSTAGHEL E, PRETORIUS E, SNOEP JL, STORBECK K. 11-Oxygenated androgen precursors are the preferred substrates for aldo-keto reductase 1C3 (AKR1C3): Implications for castration resistant prostate cancer. Journal of Steroid Biochemistry and Molecular Biology 2018; 183:192-201.
BURGER H-M, ABEL S, GELDERBLOM WCA. Modulation of key lipid raft constituents in primary rat hepatocytes by fumonisin B1- Implications for cancer promotion in the liver. FOOD AND CHEMICAL TOXICOLOGY 2018; 115:34-41.
CHRISTENSEN C, HOFMEYR JS, ROHWER JM. Delving deeper: Relating the behaviour of a metabolic system to the properties of its components using symbolic metabolic control analysis. PLoS One 2018; 13(11):e0207983 (24 pages).
CHRISTENSEN C, HOFMEYR JS, ROHWER JM. PySCeSToolbox: a collection of metabolic pathway analysis tools. Bioinformatics 2018; 34(1):124-125.
DE BEER DM, BOTES M, CLOETE TE. The microbial community of a biofilm contact reactor for the treatment of winery wastewater. Journal of Applied Microbiology 2018; 124(2):598-610.
DU TOIT A, DE WET S, HOFMEYR JS, MULLER-NEDEBOCK KK, LOOS B. The Precision Control of Autophagic Flux and Vesicle Dynamics - A Micropattern Approach. Cells 2018; 7(8):94, 17 pages.
DU TOIT A, HOFMEYR JS, GNIADEK TJ, LOOS B. Measuring autophagosome flux. Autophagy 2018; 14(6):1060-1071.
DU TOIT T, FINKEN MJJ, HAMER HM, HEIJBOER AC, SWART AC. C11-oxy C19and C11-oxy C21steroids in neonates: UPC2-MS/MS quantification of plasma 11β-hydroxyandrostenedione, 11-ketotestosterone and 11-ketoprogesterone. STEROIDS 2018; 138:1-5.
DU TOIT T, STANDER MA, SWART AC. A high-throughput UPC2-MS/MS method for the separation and quanti?cation of C19and C21steroids and their C11-oxy steroid metabolites in the classical, alternative, backdoor and 11OHA4 steroid pathways. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 2018; 1080:71-81.
DU TOIT T, SWART AC. Inefficient UGT-conjugation of adrenal 11?-hydroxyandrostenedione metabolites highlights C11-oxy C19steroids as the predominant androgens in prostate cancer. Molecular and Cellular Endocrinology 2018; 461:265-276.
GUAN J, BARNARD L, CRESSON J, HOEGL A, CHANG JH, STRAUSS E, AUCLAIR K. Probing the ligand preferences of the three types of bacterial pantothenate kinase. Bioorganic and Medicinal Chemistry 2018; 26:5896-5902.
HALLING PJ, FITZPATRICK PF, RAUSHEL FM, ROHWER JM, SCHNELL S, WITTIG U, WOHLGEMUTH R, KETTNER C. An empirical analysis of enzyme function reporting for experimental reproducibility: Missing/incomplete information in published papers. BIOPHYSICAL CHEMISTRY 2018; 242:22-27.
HAMER HM, FINKEN MJJ, VAN HERWAARDEN AE, DU TOIT T, SWART AC, HEIJBOER AC. Falsely elevated plasma testosterone concentrations in neonates: Importance of LC-MS/MS measurements. CLINICAL CHEMISTRY AND LABORATORY MEDICINE 2018; 56(6):e141-e143.
HOFMEYR JS. Causation, constructors and codes. BIOSYSTEMS 2018; 164:121-127.
HOFMEYR JS. The first special issue on code biology - A bird's-eye view. BIOSYSTEMS 2018; 164:11-15.
JENKINSON C, TAYLOR AE, STORBECK K, HEWISON M. Analysis of multiple vitamin D metabolites by ultra-performance supercritical fluid chromatography-tandem mass spectrometry (UPSFC-MS/MS). JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 2018; 1087-1088:43-48.
JENKINSON C, TAYLOR AE, STORBECK K, HEWISON M. Data comparing the separation and elution of vitamin D metabolites on an ultra performance supercritical fluid chromatography tandem-mass spectrometer (UPSFC-MS/MS) compared to liquid chromatography (LC) and data presenting approaches to UPSFC method optimization. Data in Brief 2018; 20:426-435.
MAGANGANA TP, STANDER MA, MAKUNGA NP. Effect of nitrogen and phosphate on in vitro growth and metabolite profiles of Steviarebaudiana Bertoni (Asteraceae). Plant Cell, Tissue and Organ Culture 2018; 134(1):141-151.
NAGHILOO S, BELLSTEDT DU, CLA?EN-BOCKHOFF R. Nectar protection in arid-adapted flowers of Zygophyllaceae-Zygophylloideae. Perspectives in plant ecology, evolution and systematics 2018; 34:37-50.
OYENIHI OR, KRYGSMAN A, VERHOOG NJD, DE BEER D, SAAYMAN M, MOUTON M, LOUW A. Chemoprevention of LA7-induced mammary tumor growth by SM6Met, a well-characterized CylcopiaExtact. Frontiers in Pharmacology 2018; 9:1-18.
PERKINS MS, LOUW-DU TOIT R, AFRICANDER DJ. Hormone therapy and breast cancer: emerging steroid receptor mechanisms. JOURNAL OF MOLECULAR ENDOCRINOLOGY 2018; 61(4):R133-R160.
SANTOLARIA C, VEL?ZQUEZ D, STRAUSS E, ARI?O J. Mutations at the hydrophobic core affect Hal3 trimer stability, reducing its Ppz1 inhibitory capacity but not its PPCDC moonlighting function. Scientific Reports 2018; 8:14701 (12 pages).
SCHIFFER L, ARLT W, STORBECK K. Intracrine androgen biosynthesis, metabolism and action revisited. Molecular and Cellular Endocrinology 2018; 465:4-26.
SHOKO R, MANASA J, MAPHOSA M, MBANGA J, MUDZIWAPASI R, NEMBAWARE V, SANYIKA WT, TINAGO T, CHIKWAMBI Z, MAWERE C, MATIMBA A, MUGUMBATE G, MUFANDAEDZA J, MULDER N, PATTERTON H. Strategies and opportunities for promoting bioinformatics in Zimbabwe. PLoS Computational Biology 2018; 14:1-12.
SMITH C, SWART AC. Aspalathus linearis(Rooibos) - a functional food targeting cardiovascular disease. Food & Function 2018; 9:5041-5058.
STORBECK K, GILLIGAN L, JENKINSON C, BARANOWSKI ES, QUANSON J, ARLT W, TAYLOR AE. The utility of ultra-high performance supercritical fluid chromatography–tandem mass spectrometry (UHPSFC-MS/MS) for clinically relevant steroid analysis. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 2018; 1085:36-41.
SWAINSTON N, BAICI A, BAKKER BM, CORNISH-BOWDEN A, FITZPATRICK PF, HALLING PJ, LEYH TS, O'DONOVAN C, RAUSHEL FM, RESCHEL U, ROHWER JM, SCHNELL S, SCHOMBURG D, TIPTON K, TSAI M-D, WESTERHOFF HV, WITTIG U, WOHLGEMUTH R, KETTNER C. STRENDA DB: enabling the validation and sharing of enzyme kinetics data. FEBS Journal 2018; 285:2193-2204.
TJHIN ET, SPRY C, SEWELL AL, HOEGL A, BARNARD L, SEXTON AE, SIDDIQUI G, HOWIESON VM, MAIER AG, CREEK DJ, STRAUSS E, MARQUEZ R, AUCLAIR K, SALIBA KJ. Mutations in the pantothenate kinase of Plasmodium falciparum confer diverse sensitivity profiles to antiplasmodial pantothenate analogues. PLoS Pathogens 2018; 14(4):e1006918 (30 pages).
VAN ROOYEN D, GENT R, BARNARD L, SWART AC. The in vitro metabolism of 11β-hydroxyprogesterone and 11-ketoprogesterone to 11-ketodihydrotestosterone in the backdoor pathway. Journal of Steroid Biochemistry and Molecular Biology 2018; 178:203-212.
VENTER PB, MULLER MM, VESTNER J, STANDER MA, TREDOUX AGJ, PASCH H, DE VILLIERS AJ. Comprehensive three-dimensional LC x LC × ion mobility spectrometry separation combined with high-resolution MS for the analysis of complex samples. Analytical Chemistry 2018; 90(19):11643-11650.?
WEGER M, DIOTEL N, WEGER BD, BEIL T, ZAUCKER A, EACHUS H, OAKES JA, DO REGO JL, STORBECK K, GUT P, STR?HLE U, RASTEGAR S, M?LLER F, KRONE N. Expression and activity profiling of the steroidogenic enzymes of glucocorticoid biosynthesis and the fdx1 co-factors in zebrafish. JOURNAL OF NEUROENDOCRINOLOGY 2018; 30:e12586 (15 pages).
WENZEL M, RAUTENBACH M, VOSLOO JA, SIERSMA T, AISENBREY CHM, ZAITSEVA E, LAUBSCHER WE, VAN RENSBURG W, BEHRENDS JC, BECHINGER B, HAMOEN LW. The multifaceted antibacterial mechanisms of the pioneering peptide antibiotics Tyrocidine and Gramicidin S. mBio 2018; 9(5):e00802-18 (20 pages).
WILKINSON L, VERHOOG NJD, LOUW A. Disease- and treatment-associated acquired glucocorticoid resistance. Endocrine Connections 2018; 7:R328-R349.
WILKINSON L, VERHOOG NJD, LOUW A. Novel role for receptor dimerization in post-translational processing and turnover of the GRα.Scientific Reports 2018; 8:14266 (17 pages).
Journal Articles (non-subsidised)?
GIESSNER
C, MILLET V, MOSTERT KJ, GENSOLLEN T, VU MANH T-P, GARIBAL M, DIEME B,
ATTAF-BOUABDALLAH N, CHASSON L, BROUILLY N, LAPRIE C, LESLUYES T, BLAY
JY, SHINTU L, MARTIN JC, STRAUSS E, GALLAND F, NAQUET P. Vnn1
pantetheinase limits the Warburg effect and sarcoma growth by rescuing
mitochondrial activity. Life Science Alliance 2018; 1(4):e201800073 (16 pages?).?
Paten???ts
LOOS B, HOFMEYR JS, PEROLD WJ. Method and device for determining autophagic flux. Patent No. P3464ZA00SC, South Africa 2018?.
???Doctorals completed
DU TOIT T. The metabolism of 11β-hydroxyandrostenedione by steroidogenic enzymes yields metabolites contributing to the androgen pool in prostate cancer. PhD, 2018. 223 pp. Promotor: SWART AC.PERKINS MS. Investigating the mechanism of action of hormones used in hormone replacement therapy via estrogen receptor subtypes and the influence of the progesterone receptor. PhD, 2018. 243 pp. Promotor: AFRICANDER DJ. Medepromotor: LOUW-DU TOIT R.
ROOS WG. An assessment of the diversity and pathogenicity of Potato Leaf Roll Virus in South Africa. PhD, 2018. 215 pp. Promotor: BELLSTEDT DU.
TAIT T. Strategies for the detection of endocrine disrupting compounds utilizing recombinant oestrogen receptor ligand-binding domains and high specificity monoclonal antibodies. PhD, 2018. 202 pp. Promotor: SWART P. Medepromotor: SWART AC.
WILKINSON L. Homologous down-regulation of the glucocorticoid receptor is influenced by the dimerization state of the receptor. PhD, 2018. 266 pp. Promotor: LOUW A. Medepromotor: VERHOOG NJD.
Masters completed
?ANDREWS CJ. Cyclopia extracts and sex hormone binding globulin (SHBG). MSc, 2018. 153 pp. Studieleier: LOUW A. Medestudieleier: VERHOOG NJD.BERGE S. Production and characterisation of analogues of the antimicrobial tyrocidine peptides with modified aromatic amino acid residues. MSc, 2018. 166 pp. Studieleier: RAUTENBACH M.
BOUWER W. Investigation and Characterisation of Dephospho-Coenzyme A kinase: A Potential Drug Target. MSc, 2018. 87 pp. Studieleier: DE VILLIERS M. Medestudieleier: STRAUSS E.
CABRAL AA. Crosstalk between the androgen receptor and progesterone receptor isoforms in breast and prostate cancer. MSc, 2018. 126 pp. Studieleier: AFRICANDER DJ. Medestudieleier: STORBECK K.
CLAASSEN H. Investigating the parts to understand the whole: Contribution of phenolic compounds to the desired estrogenic activity of SM6Met fractions from Cyclopia subternata . MSc, 2018. 165 pp. Studieleier: LOUW A. Medestudieleier: VISSER JAK.
GENT R. An investigation into the catalytic activity of 11β-hydroxysteroid dehydrogenase towards C11-oxy C19 and C11-oxy C21 steroids. MSc, 2018. 165 pp. Studieleier: SWART AC.
JOHANNES ID. The in vitro inhibition of adrenal steroidogenic enzymes and modulation of adrenal hormone production by Sceletium tortuosum . MSc, 2018. 90 pp. Studieleier: SWART AC. Medestudieleier: SMITH C.
RAS TA. Bioinformatic characterisation of genes associated with coenzyme A biosynthesis in mycoplasmas and expression and isolation of dephospho-coenzyme A kinase from Mycoplasma sp. Ms02. MSc, 2018. 148 pp. Studieleier: BOTES A. Medestudieleier: STRAUSS E.
SWANEPOEL CJ. A systematic investigation into the quantitative effect of pH changes on the upper glycolytic enzymes of Escherichia coli and Saccharomyces cerevisiae. MSc, 2018. 120 pp. Studieleier: ROHWER JM.
VAN DYK L. Combinatorial treatments of tamoxifen with SM6Met, a selective estrogen receptor subtype modulator (SERSM), from Cyclopia subternata are superior to current endocrine treatments in breast cancer cell models. MSc, 2018. 148 pp. Studieleier: LOUW A. Medestudieleier: VERHOOG NJD.?
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Publications in 2017
Journal Articles (subsidised)
ALBERTS JF, LILLY M, RHEEDER JP, BURGER H-M, SHEPHARD GS, GELDERBLOM WCA. Technological and community-based methods to reduce mycotoxin exposure. FOOD CONTROL 2017; 73:101-109.
BARNARD L, GENT R, VAN ROOYEN D, SWART AC. Adrenal C11-oxy C21 steroids contribute to the C11-oxy C19 steroid pool via the backdoor pathway in the biosynthesis and metabolism of 21-deoxycortisol and 21-deoxycortisone. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY 2017; 174:86-95.
BOTES A, HORNE H, PARSONS N, BELLSTEDT DU. Conservation implications of avian malaria exposure for African penguins during rehabilitation. South African Journal of Science 2017; 113(7/8):Art.#2016-0244, 8 pages.
DE VILLIERS M, SPRY C, MACUAMULE CJ, BARNARD L, WELLS G, SALIBA KJ, STRAUSS E. Antiplasmodial Mode of Action of Pantothenamides: Pantothenate Kinase Serves as a Metabolic Activator Not as a Target. ACS Infectious Diseases 2017; 3:527-541.
DI MEO I, COLOMBELLI C, SRINIVASAN B, DE VILLIERS M, HAMADA J, JEONG SY, FOX R, WOLTJER RL, TEPPER PG, LAHAYE LL, RIZZETTO E, ET AL . Acetyl-4′-phosphopantetheine is stable in serum and prevents phenotypes induced by pantothenate kinase deficiency. Scientific Reports 2017; 7:11260, 12 pages.
DU PREEZ LL, PATTERTON H. The effect of epigenetic modifications on the secondary structures and possible binding positions of the N-terminal tail of histone H3 in the nucleosome: a computational study. JOURNAL OF MOLECULAR MODELING 2017; 23:137, 15 pages.
DU TOIT T, BLOEM LM, QUANSON J, EHLERS R, SERAFIN AM, SWART AC. Profiling adrenal 11β-hydroxyandrostenedione metabolites in prostate cancer cells, tissue and plasma: UPC2-MS/MS quantification of 11β-hydroxytestosterone, 11keto-testosterone and 11keto-dihydrotestosterone. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY 2017; 166:54-67.
EACHUS H, ZAUCKER A, OAKES JA, GRIFFIN A, WEGER M, G?RAN T, TAYLOR AE, HARRIS A, GREENFIELD A, QUANSON J, STORBECK K, CUNLIFFE VT, M?LLER F. Genetic Disruption of 21-Hydroxylase in Zebrafish Causes Interrenal Hyperplasia. ENDOCRINOLOGY 2017; 158(12):4165-4173.
FIGUEIREDO AS, KOURIL T, ESSER D, HAFERKAMP P, WIELOCH P, SCHOMBURG D, RUOFF P, SIEBERS B, SCHABER J. Systems biology of the modified branched Entner-Doudoroff pathway in Sulfolobus solfataricus. PLoS One 2017; 12(7):e0180331, 25 pages.
GELDERBLOM WCA, JOUBERT E, GAMIELDIEN K, SISSING L, MALHERBE CJ, MARITZ G. Rooibos (Aspalathus linearis), honeybush (Cyclopia intermedia) and cancer bush (Sutherlandia frutescens subsp. microphylla) protect against tobacco-specific mutagenesis in vitro. SOUTH AFRICAN JOURNAL OF BOTANY 2017; 110:194-200.
GOOSEN R, STRAUSS E. Simultaneous quantification of coenzyme A and its salvage pathway intermediates in in vitro and whole cell-sourced samples. RSC Advances2017; 7:19717-19724.
HAANSTRA J, GERDING A, DOLGA AM, SORGDRAGER FJH, BUIST-HOMAN M, DU TOIT F, FABER KN, HOLZH?TTER H-G, SZ??R B, MATTHEWS R, SNOEP JL, WESTERHOFF HV, BAKKER BM. Targeting pathogen metabolism without collateral damage to the host. Scientific Reports 2017; 7:40406, 15 pages.
HAYWARD S, CILLIERS FP, SWART P. Lipoxygenases: From Isolation to Application. COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY 2017; 16:199-211.
HOFMEYR JS. Mathematics and biology. South African Journal of Science 2017; 113:Art. #a0203, 3 pages.
JONES CM, MALLAPPA A, REISCH N, NIKOLAOU N, KRONE N, HUGHES BA, O'NEIL DM, WHITAKER MJ, TOMLINSON JW, STORBECK K, MERKE DP, ROSS J, ARLT W. Modified-Release and Conventional Glucocorticoids and Diurnal Androgen Excretion in Congenital Adrenal Hyperplasia. Journal of Clinical Endocrinology and Metabolism 2017; 102(6):1797-1806.
KADLEC M, BELLSTEDT DU, LE MAITRE NC, PIRIE MD. Targeted NGS for species level phylogenomics: "made to measure'' or "one size fits all''?. PeerJ 2017; 5:e3569, 25 pages.
KOURIL T, EICHER JJ, SIEBERS B, SNOEP JL. Phosphoglycerate kinase acts as a futile cycle at high temperature. MICROBIOLOGY 2017; 163:1604-1612.
LOUW-DU TOIT R, PERKINS MS, HAPGOOD JP, AFRICANDER DJ. Comparing the androgenic and estrogenic properties of progestins used in contraception and hormone therapy. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2017; 491:140-146.
LOUW-DU TOIT R, STORBECK K, CARTWRIGHT M, CABRAL AA, AFRICANDER DJ. Progestins used in endocrine therapy and the implications for the biosynthesis and metabolism of endogenous steroid hormones. MOLECULAR AND CELLULAR ENDOCRINOLOGY 2017; 441:31-45.
MAREE JP, POVELONES ML, CLARK DJ, RODENKO G, PATTERTON H. Well?positioned nucleosomes punctuate polycistronic pol II transcription units and flank silent VSG gene arrays in Trypanosoma brucei. Epigenetics and Chromatin 2017; 10:14, 21 pages.
MCMURRY JA, JUTY N, BLOMBERG N, BURDETT T, CONLIN T, CONTE N, COURTOT M, DECK J, DUMONTIER M, FELLOWS DK, SNOEP JL, ET AL . Identifiers for the 21st century: How to design, provision, and reuse persistent identifiers to maximize utility and impact of life science data. PLOS BIOLOGY 2017; 15(6):e2001414, 18 pages.
NAGY B, WATTERS BR, VAN DER MERWE PDW, BELLSTEDT DU, COTTERILL FPD. Nothobranchius cooperi (Teleostei: Cyprinodontiformes): a new species of annual killifish from the Luapula River drainage, northern Zambia. African Journal Of Aquatic Science 2017; 42(3):201-218.
NAGY B, WATTERS BR, VAN DER MERWE PDW, COTTERILL FPD, BELLSTEDT DU. Nothobranchius cooperi (Teleostei: Cyprinodontiformes): a new species of annual killifish from the Luapula River drainage, northern Zambia. African Journal Of Aquatic Science 2017; 42(3):201-218.
NDLOVU T, RAUTENBACH M, KHAN S, KHAN W. Variants of lipopeptides and glycolipids produced by Bacillus amyloliquefaciens and Pseudomonas aeruginosa cultured in different carbon substrates. AMB Express 2017; 7:109, 13 pages.
NDLOVU T, RAUTENBACH M, VOSLOO JA, KHAN S, KHAN W. Characterisation and antimicrobial activity of biosurfactant extracts produced by Bacillus amyloliquefaciensand Pseudomonas aeruginosa isolated from a wastewater treatment plant. AMB Express 2017; 7:108, 19 pages.
O'REILLY MW, KEMPEGOWDA P, JENKINSON C, TAYLOR AE, QUANSON J, STORBECK K, ARLT W. 11-Oxygenated C19 Steroids Are the Predominant Androgens in Polycystic Ovary Syndrome. Journal of Clinical Endocrinology and Metabolism 2017; 102(3):840-848.
PERKINS MS, LOUW-DU TOIT R, AFRICANDER DJ. A comparative characterization of estrogens used in hormone therapy via estrogen receptor (ER)-α and -β. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY 2017; 174:27-39.
PETERS M, EICHER JJ, VAN NIEKERK DD, WALTEMATH D, SNOEP JL. The JWS online simulation database. BIOINFORMATICS 2017; 33(10):1589-1590.
PIRIE KR, OLIVER EGH, GEHRKE B, MUGRABI DE KUPPLER AL, LE MAITRE NC, BELLSTEDT DU. Underestimated regional species diversity in the Cape Floristic Region revealed by phylogenetic analysis of the Erica abietina/E. viscaria clade (Ericaceae). BOTANICAL JOURNAL OF THE LINNEAN SOCIETY 2017; 2(184):185–203.
PRETORIUS E, ARLT W, STORBECK K. A new dawn for androgens: Novel lessons from 11-oxygenated C19 steroids. MOLECULAR AND CELLULAR ENDOCRINOLOGY 2017; 441:76-85.
RAUTENBACH M, VLOK NM, EYEGHE-BICKONG H, STANDER MA. An Electrospray Ionization Mass Spectrometry Study on the "In Vacuo" Hetero-Oligomers Formed by the Antimicrobial Peptides, Surfactin and Gramicidin S. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY 2017; 28:1623-1637.
SERGEANT CA, AFRICANDER DJ, SWART P, SWART AC. Sutherlandia frutescens modulates adrenal hormone biosynthesis, acts as a selective glucocorticoid receptor agonist (SEGRA) and displays antimineralocorticoid properties. JOURNAL OF ETHNOPHARMACOLOGY 2017; 202:290-301.
SNYMAN MA, STORBECK K, SWART P. Evaluation of production and reproduction of three South African Angora goat CYP17 genotypes. South African Journal of Animal Sciences 2017; 47(4):478-493.
STANDER MA, VAN WYK B-E, TAYLOR MJC, LONG HS. Analysis of Phenolic Compounds in Rooibos Tea (Aspalathus linearis) with a Comparison of Flavonoid-Based Compounds in Natural Populations of Plants from Different Regions. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2017; 65:10270-10281.
TSHILILO NO, STRAZZULLI A, COBUCCI-PONZANO B, MAURELLI L, IACONO R, BEDINI E, CORSARO MM, STRAUSS E, MORACCI M. The α-Thioglycoligase Derived from a GH89 α-N-Acetylglucosaminidase Synthesises α-N-Acetylglucosamine-Based Glycosides of Biomedical Interest. Advanced Synthesis and Catalysis 2017; 359:663-676.
VAN DER MERWE JD, DE BEER D, SWANEVELDER S, JOUBERT E, GELDERBLOM WCA. Dietary exposure to honeybush (Cyclopia) polyphenol-enriched extracts altered redox status and expression of oxidative stress and antioxidant defence-related genes in rat liver. SOUTH AFRICAN JOURNAL OF BOTANY 2017; 110:230-239.
VAN ROOYEN D, DU TOIT T, LOUW-DU TOIT R, AFRICANDER DJ, SWART P, SWART AC. The metabolic fate and receptor interaction of 16α-hydroxyprogesterone and its 5α-reduced metabolite, 16α-hydroxydihydroprogesterone. MOLECULAR AND CELLULAR ENDOCRINOLOGY 2017; 441:86-98.
VAN WYK B-E, STANDER MA, LONG HS. Senecio angustifolius as the major source of pyrrolizidine alkaloid contamination of rooibos tea (Aspalathus linearis). SOUTH AFRICAN JOURNAL OF BOTANY 2017; 110:124-131.
VAN ZIJL MC, ANECK-HAHN NH, SWART P, HAYWARD S, GENTHE B, DE JAGER C. Estrogenic activity, chemical levels and health risk assessment of municipal distribution point water from Pretoria and Cape Town, South Africa. CHEMOSPHERE 2017; 186:305-313.
VOSLOO JA, BEIMS H, ALLSOPP MH, VAN RENSBURG W, VON DER OHE W, STEINERT M, RAUTENBACH M. Tolerance of honey bee adults and larvae toward tyrothricin peptides derived from Brevibacillus parabrevis. APIDOLOGIE 2017; 48:833-844.
WOLSTENCROFT K, KREBS O, SNOEP JL, STANFORD NJ, BACALL F, GOLEBIEWSKI M, KUZYAKIV R, NGUYEN Q, OWEN S, SOILAND-REYES S, STRASZEWSKI J, VAN NIEKERK DD, WILLIAMS AR, MALMSTR?M L, RINN B, M?LLER W, GOBLE C. FAIRDOMHub: a repository and collaboration environment for sharing systems biology research. NUCLEIC ACIDS RESEARCH 2017; 45:D404-D407.
ZHANG Y, KOURIL T, SNOEP JL, SIEBERS B, BARBERIS M, WESTERHOFF HV. The Peculiar Glycolytic Pathway in Hyperthermophylic Archaea: Understanding Its Whims by Experimentation In Silico. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2017; 18:art no 876.
Chapters in Books
BELLSTEDT DU, GLAIS L, DAVIE K, LACOMME C. Evolution and origen of PVY. In: Lacomme C, Glais L, BELLSTEDT DU, Dupuis B, Karasev AV, Jacquot E (eds.) Potato virus Y: biodiversity, pathogenicity, epidemiology and management, Springer, Cham, Switzerland, 2017: 77-101.
GLAIS L, BELLSTEDT DU, LACOMME C. Diversity, characterisation and classification of PVY. In: Lacomme C, Glais L, BELLSTEDT DU, Dupuis B, Karasev AV, Jacquot E (eds.) Potato virus Y: biodiversity, pathogenicity, epidemiology and management, Springer, Cham, Swaziland, 2017: 43-76.
HOFMEYR JS. Basic Biological Anticipation. In: Poli R (ed.) Handbook of Anticipation, Springer International Publishing AG, Cham, Switzerland, 2017: 1-15.
HOFMEYR JS. Exploring the Metabolic Marketplace Trough the lens of Systems Biology. In: Green S (ed.) Philosophy of Systems Biology, History, Philosophy and Theory of the Life Sciences, Springer International Publishing AG, Cham, Switzerland, 2017: 117-124.?
???Doctorals completed
HAYWARD S. An investigation into the effects of lipoxygenase activity in dough used during the manufacturing of bread. PhD, 2017. 152 pp. Promotor: SWART P. Medepromotor: CILLIERS FP.
LE MAITRE NC. Phylogenetics of the genus Erica and anthocyanin synthesis gene expression in Erica plukenetii. PhD, 2017. 220 pp. Promotor: BELLSTEDT DU. Medepromotor: Pirie M.
SERGEANT CA. The influence of Sutherlandia frutescens on adrenal steroid hormones and downstream receptor interactions. PhD, 2017. 217 pp. Promotor: SWART AC. Medepromotor: AFRICANDER DJ.
TSHILILO NO. Engineering and application of glycosidase-derived biocatalysts in the study of mycothiol pathway enzymes. PhD, 2017. 187 pp. Promotor: STRAUSS E. Medepromotor: Moracci M.
WILKINSON L. Homologous down-regulation of the glucocorticoid receptor is influenced by the dimerization state of the receptor. PhD, 2017. 272 pp. Promotor: LOUW A. Medepromotor: VERHOOG NJD.
Masters completed
BARNARD L. The Biosynthesis of Adrenal C11-Oxy C21 Steroids, Implicated in 21-Hydroxylase Deficiency - Desoxycortisol and Desoxycortisone and Their Downstream Metabolism. MSc, 2017. 105 pp. Studieleier: SWART AC.
BARNARD M. The characterization of 17β-hydroxysteroid dehydrogenase type 2 (17βHSD2) activity towards novel C19 substrates. MSc, 2017. 104 pp. Studieleier: STORBECK K.
BARRY CJ. Modelling the glucocorticoid receptor dimerisation cycle. MSc, 2017. 123 pp. Studieleier: ROHWER JM. Medestudieleier: LOUW A.
BURGER R. Flux balance analysis of Plasmodium falciparum growth and energy metabolism. MSc, 2017. 100 pp. Studieleier: SNOEP JL. Medestudieleier: EICHER JJ.
JOHNSTONE E. Comparative secretome analysis of normal prostate and prostate cancer cell models. MSc, 2017. 134 pp. Studieleier: STORBECK K. Medestudieleier: VLOK NM.
JONKER HI. Evaluation of DNA vaccines against Mycoplasma nasistruthionis sp. nov. str. Ms03 infections in ostriches and the production of IgA heavy chain proteins. MSc, 2017. 148 pp. Studieleier: BOTES A. Medestudieleier: BELLSTEDT DU.
KELBE LS. The Effect of Salsola Tuberculatiformis Bostchantzev on the Androgen and Glucocorticoid Receptors in Prostate Cancer Cell Lines. MSc, 2017. 179 pp. Studieleier: SWART P. Medestudieleier: SWART AC.
KUTU V. Biochemical and Genetic Characterization of Bacteria Isolated From Diseased Rainbow Trout (Oncorhynchus mykiss) Farmed In Lesotho and Mpumalanga Province of South Africa. MSc, 2017. 160 pp. Studieleier: BELLSTEDT DU. Medestudieleier: Macey B, Mouton A.
MUVIRIMI TK. Comparative secretome analysis of benign prostate hyperplasia and prostate cancer cell models. MSc, 2017. 104 pp. Studieleier: STORBECK K. Medestudieleier: VLOK NM.
OLIVIER DW. The age-old problem of pollution, its role in endocrine disruption and the current analytical technologies that can be employed to monitor and assess waste water treatment plants. MSc, 2017. 173 pp. Studieleier: WOLFAARDT GM. Medestudieleier: SWART P.
OOSTHUIZEN MM. Modelling of reinvasion e?ciency in Plasmodium falciparum cultures. MSc, 2017. 83 pp. Studieleier: SNOEP JL. Medestudieleier: VAN NIEKERK DD.
SCHEEPERS M. Investigation of Coenzyme A levels in Plasmodium falciparum to ascertain the mode of action of new antimalarial candidates. MSc, 2017. 90 pp. Studieleier: DE VILLIERS M. Medestudieleier: STRAUSS E.
SWART L. The interaction of the isomers of 2-(4-acetoxyphenyl)-2-chloro-N-methylethylammonium chloride (Compound A) and the natural products from the shrub Salsola tuberculatisformis Botsch. MSc, 2017. 133 pp. Studieleier: SWART P. Medestudieleier: SWART AC.
VAN DER MEER Y. Investigating progesterone and estrogen receptor crosstalk in breast cancer. MSc, 2017. 136 pp. Studieleier: AFRICANDER DJ. Medestudieleier: LOUW-DU TOIT R.
VAN ROOYEN D. The Metabolism Of Adrenal 11β-Hydroxyprogesterone, 11Keto-Progesterone and 16α-Hydroxyprogesterone by Steroidogenic Enzymes. MSc, 2017. 144 pp. Studieleier: SWART AC. Medestudieleier: SWART P.
VAN WYK L. An investigation into the Potato leafroll virus problem in the Sandveld region, South Africa. MSc, 2017. 197 pp. Studieleier: BELLSTEDT DU.
?
Publications in 2016
??Journal Articles (subsidised)
DU TOIT T, SWART AC. Perspective on the regulatory role of UGT2B28 as a conjugating enzyme in the progression of prostate cancer. Translational Cancer Research 2016; 5(Suppl 7):S1496-S1502.
GEHRKE B, KANDZIORA M, PIRIE MD. The evolution of dwarf shrubs in alpine environments: a case study of Alchemilla in Africa. ANNALS OF BOTANY 2016; 117:121-131.
HUGHES SJ, BARNARD L, MOTTAGHI K, TEMPEL W, ANTOSHCHENKO T, HONG BS, ALLALI-HASSANI A, SMIL D, VEDADI M, STRAUSS E, PARK H-W. Discovery of potent pantothenamide inhibitors of Staphylococcus aureus pantothenate kinase through a minimal SAR Study: Inhibition is due to trapping of the product. ACS Infectious Diseases 2016; 2:627-641.
KADEREIT JW, ALBACH DC, EHRENDORFER F, GALBANY-CASALS M, GARCIA-JACAS N, GEHRKE B, KADEREIT G, KILIAN N, KLEIN JT, KOCH MA, KROPF M, OBERPRIELER C, PIRIE MD, RITZ CM, R?–SER M, SPALIK K, SUSANNA A, WEIGEND M, WELK E, WESCHE K, ZHANG L-B, DILLENBERGER MS. Which changes are needed to render all genera of the German flora monophyletic?. Willdenowia 2016; 46(1):39-91.
LAUTERBACH M, VAN DER MERWE PDW, KE??LER L, PIRIE MD, BELLSTEDT DU, KADEREIT G. Evolution of leaf anatomy in arid environments ?�" A case study in southern African Tetraena and Roepera (Zygophyllaceae). MOLECULAR PHYLOGENETICS AND EVOLUTION 2016; 97:129-144.
LOUW-DU TOIT R, PERKINS MS, SNOEP JL, STORBECK K, AFRICANDER DJ. Fourth-generation progestins inhibit 3β-hydroxysteroid dehydrogenase type 2 and modulate the biosynthesis of endogenous steroids. PLoS One 2016; 11(10):e0164170, 23 pages.
MAGCWEBEBA TU, RIEDEL S, SWANEVELDER S, SWART P, DE BEER D, JOUBERT E, GELDERBLOM WCA. The potential role of polyphenols in the modulation of skin cell viability by Aspalathus linearis and Cyclopia spp. herbal tea extracts in vitro. JOURNAL OF PHARMACY AND PHARMACOLOGY 2016; 68:1440-1453.
MAGCWEBEBA TU, SWART P, SWANEVELDER S, JOUBERT E, GELDERBLOM WCA. Anti-inflammatory effects of Aspalathus linearis and Cyclopia spp. extracts in a UVB/keratinocyte (HaCaT) model utilising interleukin-1α accumulation as biomarker. MOLECULES 2016; 21:1323, 21 pages.
MAGCWEBEBA TU, SWART P, SWANEVELDER S, JOUBERT E, GELDERBLOM WCA. In vitro chemopreventive properties of green tea, rooibos and honeybush extracts in skin cells. MOLECULES 2016; 21:1622, 18 pages.
MULDER N, CHRISTOFFELS A, DE OLIVEIRA T, GAMIELDIEN J, HAZELHURST S, JOUBERT F, KUMUTHINI J, PILLAY CS, SNOEP JL, BISHOP OT, TIFFIN N. The development of computational biology in South Africa: Successes achieved and lessons learnt. PLoS Computational Biology 2016; 12(2):e1004395, 15 pages.
NAGY B, COTTERILL FPD, BELLSTEDT DU. Nothobranchius sainthousei, a new species of annual killifish from the Luapula River drainage in northern Zambia (Teleostei: Cyprinodontiformes). ICHTHYOLOGICAL EXPLORATION OF FRESHWATERS 2016; 27(3):233-254.
PILLAY CS, EAGLING BD, DRISCOLL SRE, ROHWER JM. Quantitative measures for redox signaling. FREE RADICAL BIOLOGY AND MEDICINE 2016; 96:290-303.
PIRIE MD, OLIVER EGH, MUGRABI DE KUPPLER AL, GEHRKE B, LE MAITRE NC, KANDZIORA M, BELLSTEDT DU. The biodiversity hotspot as evolutionary hot-bed: spectacular radiation of Erica in the Cape Floristic Region. BMC EVOLUTIONARY BIOLOGY 2016; 16:190, 11 pages.
PRETORIUS E, AFRICANDER DJ, VLOK NM, PERKINS MS, QUANSON J, STORBECK K. 11-Ketotestosterone and 11-ketodihydrotestosterone in castration resistant prostate cancer: Potent androgens which can no longer be ignored. PLoS One 2016; 11(7):e0159867, 17 pages.
QUANSON J, STANDER MA, PRETORIUS E, JENKINSON C, TAYLOR AE, STORBECK K. High-throughput analysis of 19 endogenous androgenic steroids by ultra-performance convergence chromatography tandem mass spectrometry. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 2016; 1031:131-138.
RAUTENBACH M, TROSKIE AM, VOSLOO JA, DATHE M. Antifungal membranolytic activity of the tyrocidines against filamentous plant fungi. BIOCHIMIE 2016; 130:122-131.
RAUTENBACH M, TROSKIE AM, VOSLOO JA. Antifungal peptides: To be or not to be membrane active. BIOCHIMIE 2016; 130:132-145.
RIEDEL S, ABEL S, BURGER H-M, VAN DER WESTHUIZEN L, SWANEVELDER S, GELDERBLOM WCA. Differential modulation of the lipid metabolism as a model for cellular resistance to fumonisin B1?�" induced cytotoxic effects in vitro. PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS 2016; 109:39-51.
SIBON OCM, STRAUSS E. Coenzyme A: to make it or uptake it?. NATURE REVIEWS MOLECULAR CELL BIOLOGY 2016; 17:605-606.
SMITH C, SWART AC. Rooibos (Aspalathus linearis) facilitates an anti-inflammatory state, modulating IL-6 and IL-10 while not inhibiting the acute glucocorticoid response to a mild novel stressor in vivo. Journal of Functional Foods 2016; 27:42-54.
SWART AC, SMITH C. Modulation of glucocorticoid, mineralocorticoid and androgen production in H295 cells by Trimesemine TM, a mesembrine-rich Sceletium extract. JOURNAL OF ETHNOPHARMACOLOGY 2016; 177:35-45.
THOMPSON GD, RICHARDSON DM, WILSON JR, BELLSTEDT DU, LE ROUX JJ. Genetic diversity and structure of the globally invasive tree, Paraserianthes lophantha subspecies lophantha, suggest an introduction history characterised by varying propagule pressure. Tree Genetics and Genomes 2016; 12(2):Article number 27, 9 pages.
VAN NIEKERK DD, PENKLER GP, DU TOIT F, SNOEP JL. Targeting glycolysis in the malaria parasite Plasmodium falciparum. FEBS Journal 2016; 283:634-646.
VISSER JAK, ZIERAU O, MACEJOV? D, GOERL F, MUDERS M, BARETTON GB, VOLLMER G, LOUW A. The phytoestrogenic Cyclopia extract, SM6Met, increases median tumor free survival and reduces tumor mass and volume in chemically induced rat mammary gland carcinogenesis. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY 2016; 163:129-135.
WOLF
J, STARK H, FAFENROT K, ALBERSMEIER A, PHAM TK, M??LLER KB, MEYER BH,
HOFFMANN L, SHEN L, ALBAUM SP, KOURIL T, SCHMIDT-HOHAGEN K,
NEUMANN-SCHAAL M, BR??SEN C, KALINOWSKI J, WRIGHT PC, ALBERS S-V,
SCHOMBURG D, SIEBERS B. A systems biology approach reveals major
metabolic changes in the thermoacidophilic archaeon Sulfolobus solfataricus in response to the carbon source L-fucose versus D-glucose. MOLECULAR MICROBIOLOGY 2016; 102(5):882-908.
Journal Articles (non-subsidised)
ROHWER JM, VILJOEN C, CHRISTENSEN C, MASHAMAITE LN, PILLAY CS. Identifying the conditions necessary for the thioredoxin ultrasensitive response. Perspectives in Science 2016; 9:53-59.
Chapters in Books
VAN NIEKERK DD, PENKLER GP, DU TOIT F, SNOEP JL, BAKKER BM, HAANSTRA J. Attacking Blood-Borne Parasites with Mathematics. In: M??ller S, Cerdan R, Radulescu O (eds.) Comprehensive Analysis of Parasite Biology. From Metabolism to Drug Discovery, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany, 2016: 513-541.
Patents
LOOS B, DU TOIT A, HOFMEYR JS. Method of determining autophagosome flux. Patent No. P3086ZA00, South Africa 2016.?
Doctorals completed
CHRISTENSEN C. Development of an Integrated Metabolic Analysis Toolbox. PhD, 2016. 190 pp. Promotor: ROHWER JM. Medepromotor: HOFMEYR JS.
DE BEER DM. A high rate biofilm contact reactor for winery wastewater treatment. PhD, 2016. 186 pp. Promotor: CLOETE TE. Medepromotor: SWART P.
GOOSEN R. A comparative analysis of CoA biosynthesis in selected organisms: a metabolite study. PhD, 2016. 174 pp. Promotor: STRAUSS E. Medepromotor: SNOEP JL.
LOOTS R. Biofilms as multifunctional surface coatings and adaptive systems: a biomimetic approach. PhD, 2016. 210 pp. Promotor: CLOETE TE. Medepromotor: SWART P.
VOSLOO JA. Optimised bacterial production and characterisation of natural antimicrobial peptides with potential application in agriculture . PhD, 2016. 239 pp. Promotor: RAUTENBACH M. Medepromotor: SNOEP JL?
Masters completed
ANDERSON C. A molecular phylogenetic study of the South American fish genus Pterophyllum. MSc, 2016. 182 pp. Studieleier: BELLSTEDT DU.
COETZEE M. Feedforward activation in metabolic systems. MSc, 2016. 129 pp. Studieleier: HOFMEYR JS. Medestudieleier: ROHWER JM.
DE WET B. Evaluation of DNA vaccines developed against Mycoplasma struthionis sp. nov. str. Ms01 in ostriches. MSc, 2016. 137 pp. Studieleier: BOTES A. Medestudieleier: BELLSTEDT DU.
DU TOIT A. Measuring and Modelling Autophagic Flux. MSc, 2016. 176 pp. Studieleier: LOOS B. Medestudieleier: HOFMEYR JS.
GWASHU A. Protective properties of rooibos (Aspalathus linearis) flavonoids on the prevention of skin cancer. MSc, 2016. 165 pp. Studieleier: GELDERBLOM WCA. Medestudieleier: LOUW A.
LAUBSCHER WE. Production, characterisation and activity of selected and novel antibiotic peptides from soil bacteria . MSc, 2016. 178 pp. Studieleier: RAUTENBACH M.
LOUW C. Comparison of progress curve analysis versus initial rate kinetics for the estimation of enzyme kinetic parameters. MSc, 2016. 152 pp. Studieleier: SNOEP JL. Medestudieleier: EICHER JJ.
NDLOVU E. Crosstalk between the androgen and estrogen receptors in breast cancer. MSc, 2016. 158 pp. Studieleier: AFRICANDER DJ. Medestudieleier: LOUW A.
PRETORIUS E. An investigation into the androgenic activity of 11-ketotestosterone and 11-ketodihydrotestosterone. MSc Biochemie, 2016. 132 pp. Studieleier: STORBECK K. Medestudieleier: SWART AC.
VAN SCHALKWYK FJ. Construction and analysis of a kinetic model of the ovine adrenal steroidogenic subsystem comprising CYP17 AND 3β-HSD. MSc, 2016. 103 pp. Studieleier: SWART P. Medestudieleier: SNOEP JL.
WALTERS N. Mathematical modelling of hypoglycaemia and lactic acidosis in the bloodstream of Plasmodium burghei infected rats; a feasibility study. MSc, 2016. 83 pp. Studieleier: SNOEP JL
Publications in 2015
??Journal Articles (subsidised)
ABRIE JA, MOLERO C, ARI?O J, STRAUSS E. Complex stability and dynamic subunit interchange modulates the disparate activities of the yeast moonlighting proteins Hal3 and Vhs3. Scientific Reports 2015; 5:15774, 17 pages.
BIGGS R, RHODE C, ARCHIBALD S, KUNENE LM, MUTANGA SS, NKUNA N, OCHOLLA PO, PHADIMA LJ. Strategies for managing complex social-ecological systems in the face of uncertainty: examples from South Africa and beyond. Ecology and Society 2015; 20(1):52, 15 pages.
BLOEM LM, STORBECK K, SWART P, DU TOIT T, SCHLOMS L, SWART AC. Advances in the analytical methodologies: Profiling steroids in familiar pathways-challenging dogmas. Journal of Steroid Biochemistry and Molecular Biology 2015; 153:80-92.
CHRISTENSEN C, HOFMEYR JS, ROHWER JM. Tracing regulatory routes in metabolism using generalised supply-demand analysis. BMC Systems Biology 2015; 9:89, 18 pages.
DE VILLIERS M, STRAUSS E. Jump-starting CoA biosynthesis. Nature Chemical Biology 2015; 11:757-758.
FISCHER J, GARDNER TA, BENNETT EM, BALVANERA P, BIGGS R, CARPENTER S, DAW TM, FOLKE C, HILL R, HUGHES TP, LUTHE T, SPIERENBURG MJ, ET AL . Advancing sustainability through mainstreaming a social-ecological systems perspective. Current Opinion in Environmental Sustainability 2015; 14:144-149.
GABRIELLI M, BUICA AS, FRACASSETTI D, STANDER MA, TIRELLI A, DU TOIT WJ. Determination of sotolon content in South African white wines by two novel HPLC-UV and UPLC-MS methods. Food Chemistry 2015; 169:180-186.
HAMANN M, BIGGS R, REYERS B. Mapping social-ecological systems: Identifying "green-loop" and "red-loop" dynamics based on characteristic bundles of ecosystem service use. Global Environmental Change-Human and Policy Dimensions 2015; 34:218-226.
HOMER-DIXON T, WALKER B, BIGGS R, CRéPIN A-S, FOLKE C, LAMBIN EF, PETERSON G, ROCKSTR?–M J, SCHEFFER M, STEFFEN W, TROELL M. Synchronous failure: the emerging causal architecture of global crisis. Ecology and Society 2015; 20(3):6, 16 pages.
HOUGH D, STORBECK K, CLOETE SWP, SWART AC, SWART P. Relative contribution of P450c17 towards the acute cortisol response: Lessons from sheep and goats. Molecular and Cellular Endocrinology 2015; 408:107-113.
KINFE HH, LONG HS, STANDER MA, VAN WYK B-E. The major phenolic compound of the roots and leaves of Rafnia amplexicaulis (Fabaceae), a liquorice substitute and traditional tea used in Cape Herbal Medicine. South African Journal of Botany 2015; 100:75-79.
LESOVAYA E, YEMELYANOV A, SWART AC, SWART P, HAEGEMAN G, BUDUNOVA I. Discovery of Compound A - a selective activator of the glucocorticoid receptor with anti-inflammatory and anti-cancer activity. Oncotarget 2015; 6(31):30730-30744.
MACUAMULE CJ, TJHIN ET, JANA CE, BARNARD L, KOEKEMOER L, DE VILLIERS M, SALIBA KJ, STRAUSS E. A Pantetheinase-Resistant Pantothenamide with Potent, On-Target, and Selective Antiplasmodial Activity. Antimicrobial Agents and Chemotherapy 2015; 59(6):3666-3668.
MAREE H, PIRIE MD, OOSTHUIZEN K, BESTER R, REES DJG, BURGER JT. Phylogenomic Analysis Reveals Deep Divergence and Recombination in an Economically Important Grapevine Virus. PLoS ONE 2015; 10(5):e0126819.
MASHAMAITE LN, ROHWER JM, PILLAY CS. The glutaredoxin mono- and di-thiol mechanisms for deglutathionylation are functionally equivalent: implications for redox systems biology. Bioscience Reports 2015; 35:e00173, 10 pages.
MORTIMER M, VISSER JAK, DE BEER D, JOUBERT E, LOUW A. Divide and conquer may not be the optimal approach to retain the desirable estrogenic attributes of the Cyclopia nutraceutical extract, SM6Met. PLoS ONE 2015; 10(7):e0132950, 22 pages.
MUGRABI DE KUPPLER AL, FAGúNDEZ J, BELLSTEDT DU, OLIVER EGH, LéON J, PIRIE MD. Testing reticulate versus coalescent origins of Erica lusitanica using aspecies phylogeny of the northern heathers (Ericeae, Ericaceae). Molecular Phylogenetics and Evolution 2015; 88:121-131.
NISHII K, HUGHES M, BRIGGS M, HASTON E, CHRISTIE F, DE VILLIERS MJ, HANEKOM T, ROOS WG, BELLSTEDT DU, MüLLER M. Streptocarpus redefined to include all Afro-Malagasy Gesneriaceae: Molecular phylogenies prove congruent with geographical distribution and basic chromosome numbers and uncover remarkable morphological homoplasies. Taxon 2015; 64(6):1243-1274.
PALMER CG, BIGGS R, CUMMING GS. Applied research for enhancing human well-being and environmental stewardship: using complexity thinking in Southern Africa. Ecology and Society 2015; 20(1):53, 4 pages.
PENKLER GP, DU TOIT F, ADAMS WW, RAUTENBACH M, PALM DC, VAN NIEKERK DD, SNOEP JL. Construction and validation of a detailed kinetic model of glycolysis in Plasmodium falciparum. FEBS Journal 2015; 282:1481-1511.
PIANO F, FRACASSETTI D, BUICA AS, STANDER MA, DU TOIT WJ, BORSA D, TIRELLI A. Development of a novel liquid/liquid extraction and ultra-performance liquid chromatography tandem mass spectrometry method for the assessment of thiols in South African Sauvignon Blanc wines. Australian Journal of Grape and Wine Research 2015; 21:40-48.
PIRIE MD, LITSIOS G, BELLSTEDT DU, SALAMIN N, KISSLING J. Back to Gondwanaland: can ancient vicariance explain (some) Indian Ocean disjunct plant distributions?. Biology Letters 2015; 11:20150086, 4 pages.
PIRIE MD. Phylogenies from concatenated data: Is the end nigh?. Taxon 2015; 64(3):421-423.
RIEDEL S, ABEL S, SWANEVELDER S, GELDERBLOM WCA. Induction of an altered lipid phenotype by two cancer promoting treatments in rat liver. Food and Chemical Toxicology 2015; 78:96-104.
ROCHA J, PETERSON G, BIGGS R. Regime Shifts in the Anthropocene: Drivers, Risks, and Resilience. PLoS ONE 2015; 10(8):e0134639, 16 pages.
ROCHA J, YLETYINEN J, BIGGS R, BLENCKNER T, PETERSON G. Marine Regime Shifts: Drivers and Impacts on Ecosystems Services. Philosophical Transactions of the Royal Society B-Biological Sciences 2015; 370:20130273, 12 pages.
SELOMANE AO, REYERS B, BIGGS R, TALLIS H, POLASKY S. Towards integrated social?�"ecological sustainability indicators: Exploring the contribution and gaps in existing global data. Ecological Economics 2015; 118:140-146.
SNOEP JL, GREEN KA, EICHER JJ, PALM DC, PENKLER GP, DU TOIT F, WALTERS N, BURGER R, WESTERHOFF HV, VAN NIEKERK DD. Quantitative analysis of drug effects at the whole-body level: a case study for glucose metabolism in malaria patients. Biochemical Society Transactions 2015; 43:1157-1163.
SPRY C, SALIBA KJ, STRAUSS E. A miniaturized assay for measuring small molecule phosphorylation in the presence of complex matrices. Analytical Biochemistry 2014; 451:76-78.
STANFORD NJ, WOLSTENCROFT K, GOLEBIEWSKI M, KANIA R, JUTY N, TOMLINSON C, OWEN S, BUTCHER S, HERMJAKOB H, LE NOVèRE N, MUELLER W, SNOEP JL, GOBLE C. The evolution of standards and data management practices in systems biology. Molecular Systems Biology 2015; 11:851, 4 pages.
STEFFEN W, RICHARDSON K, ROCKSTR?M J, CORNELL SE, FETZER I, BENNETT EM, BIGGS R, CARPENTER S, DE VRIES W, DE WIT CA, FOLKE C, GERTEN D, HEINKE J, MACE GM, PERSSON LM, RAMANATHAN V, REYERS B, S?RLIN S. Planetary boundaries: Guiding human development on a changing planet. Science 2015; 347(6223):1259855, 10 pages.
STORBECK K, SWART AC, FOX C, SWART P. Cytochrome b5 modulates multiple reactions in steroidogenesis by diverse mechanisms. Journal of Steroid Biochemistry and Molecular Biology 2015; 151:66-73.
SWART AC, STORBECK K. 11β-hydroxyandrostenedione: Downstream metabolism by 11βHSD, 17βHSD and SRD5A produces novel substrates in familiar pathways. Molecular and Cellular Endocrinology 2015; 408:114-123.
THOMPSON GD, BELLSTEDT DU, RICHARDSON DM, WILSON JR, LE ROUX JJ. A tree well travelled: global genetic structure of the invasive tree Acacia saligna. Journal of Biogeography 2015; 42:305-314.
VAN DER MERWE JD, DE BEER D, JOUBERT E, GELDERBLOM WCA. Short-term and sub-chronic dietary exposure to aspalathin-enriched green rooibos (Aspalathus linearis) extract affects rat liver function and antioxidant status. Molecules 2015; 20:22674-22690.
WILLEMSE CM, STANDER MA, VESTNER J, TREDOUX AGJ, DE VILLIERS AJ. Comprehensive two-dimensional hydrophilic interaction chromatography (HILIC) X reversed-phase liquid chromatography coupled to high-resolution mass spectrometry (RP-LC-UV-MS) analysis of anthocyanins and derived pigments in red wine. Analytical Chemistry 2015; 87:12006-12015.
WIUM M, BOTES A, BELLSTEDT DU. The identification of oppA gene homologues as part of the oligopeptide transport system in mycoplasmas. Gene 2015; 558:31-40.
WOLSTENCROFT
K, OWEN S, KREBS O, NGUYEN Q, STANFORD NJ, GOLEBIEWSKI M, WEIDEMANN A,
BITTKOWSKI M, AN L, SHOCKLEY D, SNOEP JL, MEULLER W, GOBLE C. SEEK: a
systems biology data and model management platform. BMC Systems Biology 2015; 9:33, 12 pages.
Journal Articles (non-subsidised)
MUGABO P , ABANIWONDA MI, THERON D, VAN ZYL L, HASSAN SM, STANDER MA, MCILLERON H, MADSEN R. Determination of Kanamycin Plasma Levels Using LC-MS and Its Pharmacokinetics in Patients with Multidrug- Resistant Tuberculosis with and without HIV-Infection. Biochemistry & Pharmacology 2015; 4(1):1000160, 7 pages.
MURABITO
E, COLOMBO R, WU C, VERMA M, REHMAN S, SNOEP JL, PENG S-L, GUAN N, LIAO
X, WESTERHOFF HV. SupraBiology 2014: Promoting UK-China collaboration
on Systems Biology and High Performance Computing. Quantitative Biology 2015; 3(1):46-53.
Chapters in Books
BIGGS R, GORDON L, RAUDSEPP-HEARNE C, SCHLüTER M, WALKER B. Principle 3: Manage slow variables and feedbacks. In: BIGGS R, Schlüter M, Schoon ML (eds.) Principles for Building Resilience: Sustaining Ecosystem Services in Social-Ecological Systems, Cambridge 中国体育彩票 Press, Cambridge, United Kingdom, 2015: 105-141.
BIGGS R, SCHLüTER M, SCHOON ML. An introduction to the resilience approach and principles to sustain ecosystem services in social-ecological systems. In: BIGGS R, Schlüter M, Schoon ML (eds.) Principles for Building Resilience: Sustaining Ecosystem Services in Social-Ecological Systems, Cambridge 中国体育彩票 Press, Cambridge, United Kingdom, 2015: 1-31.
KOTSCHY K, BIGGS R, DAW TM, FOLKE C, WEST PC. Principle 1: Maintain diversity and redundancy. In: BIGGS R, Schlüter M, Schoon ML (eds.) Principles for Building Resilience: Sustaining Ecosystem Services in Social-Ecological Systems, Cambridge 中国体育彩票 Press, Cambridge, United Kingdom, 2015: 50-79.
SCHLüTER M, BIGGS R, SCHOON ML, ROBARDS MD, ANDERIES JM. Reflections on building resilience - interactions among principles and implications for governance. In: BIGGS R, Schlüter M, Schoon ML (eds.) Principles for Building Resilience: Sustaining Ecosystem Services in Social-Ecological Systems, Cambridge 中国体育彩票 Press, Cambridge, United Kingdom, 2015: 251-282.
SCHOON ML, ROBARDS MD, BROWN K, ENGLE N, MEEK C, BIGGS R. Politics and the resilience of ecosystem services. In: BIGGS R, Schlüter M, Schoon ML (eds.) Principles for Building Resilience: Sustaining Ecosystem Services in Social?�"Ecological Systems, Cambridge 中国体育彩票 Press, Cambridge, United Kingdom, 2015: 32-49.
VISSER
JC, BELLSTEDT DU. Potato Virus Y in South Africa: Isolate
Characterization and Assessment of Potato Cultivar Resistance. In: Low
J, Nyongesa M, Quinn S, Parker M (eds.) Potato and Sweetpotato in Africa: Transforming the Value Chains for Food and Nutrition Security, CABI, OXFORDSHIRE, United Kingdom, 2015: 279-288.
Patents
RAUTENBACH M, DE BEER A, TROSKIE AM, VOSLOO JA. Antimicrobial peptide compositions for plants. Patent No. WO/2013/150394, South Africa 2015.
???Doctorals completed
DU TOIT F. Modeling glycolysis in Plasmodium-infected erythrocytes. PhD(Bioch), 2015. 202 pp. Promotor: SNOEP JL.
MOOLMAN WJA. Coenzyme A biosynthesis and Coenzyme A-dependent redox processes as targets for anti-staphylococcal drug development. PhD(Bioch), 2015. 149 pp. Promotor: STRAUSS E.
SCHLOMS L. The inhibition of adrenal steroidogenic enzymes and modulation of glucocorticoid levels in vitro and in vivo by Aspalathus linearis (Rooibos). PhD(Bioch), 2015. 122 pp. Promotor: SWART AC. Medepromotor: STORBECK K, SMITH C.
WIUM M. The development of a DNA vaccine against Mycoplasma nasistruthionis sp. nov. for use in ostriches. PhD(Bioch), 2015. 271 pp. Promotor: BOTES A. Medepromotor: BELLSTEDT DU.
Masters completed
ADAMS WW. Glycerol production in Plasmodium falciparum: Towards a detailed kinetic model. MSc Biochemie, 2015. 104 pp. Studieleier: SNOEP JL. Medestudieleier: RAUTENBACH M.DE LA HARPE A. A Comparative Analysis of Mathematical Models for HIV Epidemiology. MSc Biochemie, 2015. 125 pp. Studieleier: SNOEP JL.
DU TOIT T. The influence of Rooibos on androgen metabolism in normal and cancer prostate cells. MSc Biochemie, 2015. 139 pp. Studieleier: SWART AC. Medestudieleier: STORBECK K.
KEET L. Development of in vitro models to investigate the anti-inflammatory properties of Cyclopia maculata and other herbal teas. MSc Biochemie, 2015. 156 pp. Studieleier: GELDERBLOM WCA. Medestudieleier: SWART AC, Riedel S.
MUGARI MB. The inhibitory effect of Rooibos on cytochromes P450 and downstream in vitro modulation of steroid hormones. MSc Biochemie, 2015. 130 pp. Studieleier: SWART AC. Medestudieleier: SWART P.
QUANSON J. The characterisation of the catalytic activity of human steroid 5alpha reductase towards novel C19 substrates. MSc Biochemie, 2015. 88 pp. Studieleier: STORBECK K. Medestudieleier: SWART AC.
TAIT T. The Production and Purification of Functional Steroid Hormone Receptor Ligand Binding Domains towards the Development of a Biological Endocrine Disruptor Detection System. MSc Biochemie, 2015. 140 pp. Studieleier: SWART P.
VAN DER MERWE PDW. Systematic and Genomic Studies in the Plant Genus Zygophyllum. MSc Biochemie, 2015. 193 pp. Studieleier: BELLSTEDT DU.
VAN RENSBURG W. Characterisation of natural antimicrobial peptides adsorbed to different matrixes. MSc Biochemie, 2015. 160 pp. Studieleier: RAUTENBACH M.
?Publications in 2014
?Journal Articles (subsidised)?
AFRICANDER D, STORBECK K-H, HAPGOOD JP. A comparative study of the androgenic properties of progesterone and the progestins, medroxyprogesterone acetate (MPA) and norethisterone acetate (NET-A). Journal of Steroid Biochemistry and Molecular Biology 2014; 143 : 404-415.
BURGER H-M, LOMBARD MJ, SHEPHARD GS, DANSTER-CHRISTIANS N, GELDERBLOM WCA. Development and Evaluation of a Sensitive Mycotoxin Risk Assessment Model (MYCORAM). Toxicological Sciences 2014; 141(2) : 387-397.
CILLIERS FP, GOUWS PA, KOUTCHMA T, ENGELBRECHT Y, ADRIAANSE C, SWART P. A microbiological, biochemical and sensory characterisation of bovine milk treated by heat and ultraviolet (UV) light for manufacturing Cheddar cheese. Innovative Food Science & Emerging Technologies 2014; 23 : 94-106.
DE VILLIERS M, BARNARD L, KOEKEMOER L, SNOEP JL, STRAUSS E. Variation in pantothenate kinase type determines the pantothenamide mode of action and impacts on coenzyme A salvage biosynthesis. FEBS Journal 2014; 281 : 4731-4753.
GAERTNER M, BIGGS R, TE BEEST M, HUI C, MOLOFSKY J, RICHARDSON DM. Invasive plants as drivers of regime shifts: identifying high-priority invaders that alter feedback relationships. Diversity and Distributions 2014; 20(7) : 733-744.
GOVENDER Y, AVENANT C, VERHOOG N, RAY RM, GRANTHAM NJ, AFRICANDER D, HAPGOOD JP. The Injectable-Only Contraceptive Medroxyprogesterone Acetate, Unlike Norethisterone Acetate and Progesterone, Regulates Inflammatory Genes in Endocervical Cells via the Glucocorticoid Receptor. PLoS ONE 2014; 9(5) : e96497.
GROBBELAAR MC, MAKUNGA NP, STANDER MA, KOSSMANN J, HILLS PN. Effect of strigolactones and auxins on growth and metabolite content of Sutherlandia frutescens (L.) R. Br. microplants in vitro. Plant Cell Tissue and Organ Culture 2014; 117 : 401-409.
GUSTAVSSON A-K, VAN NIEKERK DD, ADIELS CB, GOKS?–R M, SNOEP JL. Heterogeneity of glycolytic oscillatory behaviour in individual yeast cells. FEBS Letters 2014; 588 : 3-7.
GUSTAVSSON A-K, VAN NIEKERK DD, ADIELS CB, KOOI BW, GOKS?R M, SNOEP JL. Allosteric regulation of phosphofructokinase controls the emergence of glycolytic oscillations in isolated yeast cells. FEBS Journal 2014; 281 : 2784-2793.
HAPGOOD JP, AFRICANDER D, LOUW R, RAY RM, ROHWER JM. Potency of progestogens used in hormonal therapy: Toward understanding differential actions. Journal of Steroid Biochemistry and Molecular Biology 2014; 142 : 39-47.
K??HN S, HOFMEYR J-HS. Is the ?�?Histone Code?� an Organic Code?. Biosemiotics 2014; 7(2) : 203-222.
LEADLEY P, PROENCA V, FERN?NDEZ-MANJARR?S J, PEREIRA HM, ALKEMADE R, BIGGS R, BRULEY E, CHEUNG W, COOPER D, FIGUEIREDO J, GILMAN E, GU?NETTE S, HURTT G, MBOW C, OBERDORFF T, REVENGA C, SCHARLEMANN JPW, SCHOLES RJ, SMITH MS, SUMAILA RU, WALPOLE M. Interacting Regional-Scale Regime Shifts for Biodiversity and Ecosystem Services. Bioscience 2014; 64(8) : 665-679.
LEUSSA AN-N, RAUTENBACH M. Detailed SAR and PCA of the Tyrocidines and Analogues Towards Leucocin A-Sensitive and Leucocin A-Resistant Listeria monocytogenes. Chemical Biology & Drug Design 2014; 84 : 543-557.
LOOS B, DU TOIT A, HOFMEYR J-HS. Defining and measuring autophagosome flux: concept and reality. Autophagy 2014; 10(11) : 2087-2096.
LOUW-DU TOIT R, HAPGOOD JP, AFRICANDER D. Medroxyprogesterone Acetate Differentially Regulates Interleukin (IL)-12 and IL-10 in a Human Ectocervical Epithelial Cell Line in a Glucocorticoid Receptor (GR)-dependent Manner. Journal of Biological Chemistry 2014; 289(45) : 31136-31149.
MACE GM, REYERS B, ALKEMADE R, BIGGS R, CHAPIN FS, CORNELL SE, DIAZ S, JENNINGS S, LEADLEY P, MUMBY PJ, PURVIS A, SCHOLES RJ, SEDDON AWR, SOLAN M, STEFFEN W, WOODWARD G. Approaches to defining a planetary boundary for biodiversity. Global Environmental Change-Human and Policy Dimensions 2014; 28 : 289-297.
MOOLMAN WJA, DE VILLIERS M, STRAUSS E. Recent advances in targeting coenzyme A biosynthesis and utilization for antimicrobial drug development. Biochemical Society Transactions 2014; 42(4) : 1080-1086.
MPHAHLELE RR, FAWOLE OA, STANDER MA, OPARA UL. Preharvest and postharvest factors influencing bioactive compounds in pomegranate (Punica granatum L.): A review. Scientia Horticulturae 2014; 178 : 114-123.
MPHAHLELE RR, STANDER MA, FAWOLE OA, OPARA UL. Effect of fruit maturity and growing location on the postharvestcontents of flavonoids, phenolic acids, vitamin C and antioxidantactivity of pomegranate juice (cv. Wonderful). Scientia Horticulturae 2014; 179 : 36-45.
NIEUWOUDT M, LOMBARD N, RAUTENBACH M. Optimised purification and characterisation of lipid transfer protein 1 (LTP1) and its lipid-bound isoform LTP1b from barley malt. Food Chemistry 2014; 157 : 559-567.
PALM DC, ROHWER JM, HOFMEYR J-HS. Incorporating covalent and allosteric effects into rate equations: the case of muscle glycogen synthase. Biochemical Journal 2014; 462 : 525-537.
SCHLOMS L, SMITH C, STORBECK K-H, MARNEWICK JL, SWART P, SWART AC. Rooibos influences glucocorticoid levels and steroid ratios in vivo and in vitro: A natural approach in the management of stress and metabolic disorders?. Molecular Nutrition & Food Research 2014; 58 : 537-549.
SCHLOMS L, SWART AC. Rooibos Flavonoids Inhibit the Activity of Key Adrenal Steroidogenic Enzymes, Modulating Steroid Hormone Levels in H295R Cells. Molecules 2014; 19 : 3681-3695.
SHEPHARD GS, GELDERBLOM WCA. Rapid testing and regulating for mycotoxin concerns: a perspective from developing countries. World Mycotoxin Journal 2014; 7(4) : 431-437.
TROSKIE AM, DE BEER A, VOSLOO JA, JACOBS K, RAUTENBACH M. Inhibition of agronomically relevant fungal phytopathogens by tyrocidines, cyclic antimicrobial peptides isolated from Bacillus aneurinolyticus . Microbiology 2014; 160 : 2089-2101.
TROSKIE AM, RAUTENBACH M, DELATTIN N, VOSLOO JA, DATHE M, CAMMUE BPA, THEVISSEN K. Synergistic Activity of the Tyrocidines, Antimicrobial Cyclodecapeptides from Bacillus aneurinolyticus, with Amphotericin B and Caspofungin against Candida albicans Biofilms. Antimicrobial Agents and Chemotherapy 2014; 58(7) : 3697-3707.
VAN WYK P, VAN DYK JB, GERBER WJ, STANDER MA, DE VILLIERS AJ, KOCH KR. Speciation of [PtIVCl6-nBrn]2- (n = 0-6) and some of their mono-aquated [PtIVCl5-nBrn(H2O)]- (n = 0-5) anions in solution at low concentrations by means of ion-pairing reversed-phase ultra-high-performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight mass spectrometry. Rapid Communications in Mass Spectrometry 2014; 28 : 505-519.
VERHOOG N, ALLIE-REID F, VANDEN BERGHE W, SMITH C, HAEGEMAN G, HAPGOOD JP, LOUW A. Inhibition of Corticosteroid-Binding Globulin Gene Expression by Glucocorticoids Involves C/EBP??. PLoS ONE 2014; 9(10) : e110702.
WILLEMSE CM, STANDER MA, TREDOUX AGJ, DE VILLIERS AJ. Comprehensive two-dimensional liquid chromatographic analysis of anthocyanins. Journal of Chromatography A 2014; 1359 : 189-201.
WRIGHT LP, ROHWER JM, GHIRARDO A, HAMMERBACHER A, ORTIZ-ALCAIDE M, RAGUSCHKE B, SCHNITZLER J-P, GERSHENZON J, PHILLIPS MA. Deoxyxylulose 5-Phosphate Synthase Controls Flux through the Methylerythritol 4-Phosphate Pathway in Arabidopsis1.
Plant Physiology 2014;
165 : 1488-1504.
Journal Articles (non-subsidised)
PAUL S, M?LLER HP, PREISER R, DE LIMA NETO FB, MARWALA T, DE WILDE P. Developing a management decision-making model based upon a complexity perspective with reference to the Bee Algorithm. Emergence: complexity and organization 2014; 16(1) : 1-12.
PREISER R, STRUTHERS P, MOHAMED S, CAMERON NA, LAWRENCE E. External stakeholders and health promoting schools: complexity and practice in South Africa. Health Education 2014; 114(4) : 260-270.
TIPTON K, ARMSTRONG R, BAKKER BM, BAIROCH A, CORNISH-BOWDEN A, HALLING PJ, HOFMEYR J-HS, LEYH TS, KETTNER C, RAUSHEL FM, ROHWER JM, SCHOMBURG D, STEINBECK C. Standards for Reporting Enzyme Data: The STRENDA Consortium: What it aims to do and why it should be helpful.
Perspectives in Science 2014;
1 : 131-137.
Chapters in Books
LOOS B, HOFMEYR JS, M?LLER-NEDEBOCK KK, BOONZAAIER L, KINNEAR CJ. Autophagic Flux, Fusion Dynamics and Cell Death. In Autophagy: Cancer, Other Pathologies, Inflammation, Immunity, Infection and Aging, Volume 3, Elsevier, USA, 2014: 39-56.
ROHWER JM, UYS L. Systems Biology and Metabolic Modeling. In SUGERCANE Physiology, Biochemistry and Functional Biology, John Wiley & Sons, Inc, , 2014: 601-622.
ROHWER JM. Applications of Kinetic Modeling to Plant Metabolism. In
Plant Metabolism Methods and protocols, Springer Science+Business Media, New York, 2014: 275-286.
Patents
RAUTENBACH M, DE BEER A, TROSKIE AM, VOSLOO JA. Antimicrobial cyclic peptide compositions for plants. Patent No. WO 2013/150394 A1, 2014.
Doctorals completed?
LEUSSA AN-N. Characterisation of small cyclic peptides with antilisterial and antimalarial activity. PhD(Bioch), 2014. 295 pp. Promotor: Rautenbach M.
MACUAMULE C. Coenzyme A biosynthesis and utilization in Plasmodium falciparum: drug targets for antimalarial chemotherapy. PhD(Bioch), 2014. 178 pp. Promotor: Strauss E. Medepromotor: Saliba KJ.
TROSKIE AM. Tyrocidines, cyclic decapeptides produced by soil bacilli, as potent inhibitors of fungal pathogens. PhD(Bioch), 2014. 215 pp. Promotor: Rautenbach M.
VISSER JAK. Phytoestrogenic extracts of Cyclopia modulate molecular targets involved in the prevention and treatment of breast cancer. PhD(Bioch), 2014. 253 pp. Promotor: Louw A.
Masters completed
FOX C-L. An investigation into the catalytic activity of porcine cytochrome P450 17α-hydroxylase/17,20-lyase . MSc Biochemie, 2014. 126 pp. Studieleier: Swart AC. Medestudieleier: Swart P, Storbeck K-H.
HAYWARD S. Partial characterization of a bacterial acyl-transferase enzyme for potential application in dairy processing. MSc Biochemie, 2014. 129 pp. Studieleier: Swart P.
KüHN S. Organic codes and their identification: Is the histone code a true organic code?. MSc Biochemie, 2014. 97 pp. Studieleier: Hofmeyr J-HS.
MORTIMER M. Isolation and identification of compounds conferring phytoestrogenic activity to Cyclopia extracts. MSc Biochemie, 2014. 310 pp. Studieleier: Louw A. Medestudieleier: Joubert E.
VAN NIEKERK C. Investigating self-fabrication in the context of artificial chemistries. MSc Biochemie, 2014. 160 pp. Studieleier: Hofmeyr J-HS.
VAN STADEN CT. Towards a kinetic model of the Entner-Doudoroff Pathway in Zymomonas mobilis. MSc Biochemie, 2014. 84 pp. Studieleier: Rohwer JM. Medestudieleier: Snoep JL.
?Publications in 2013
?Journal Articles (subsidised)?
AFRICANDER D, LOUW R, HAPGOOD JP. Investigating the anti-mineralocorticoid properties of synthetic progestins used in hormone therapy. Biochemical and Biophysical Research Communications 2013; 433 : 305-310.
ALBERTS P, KIDD M, STANDER MA, NIEUWOUDT HH, TREDOUX AGJ, DE VILLIERS AJ. Quantitative survey of 3-alkyl-2-methoxypyrazines and first confirmation of 3-ethyl-2-methoxypyrazine in South African Sauvignon blanc wines. South African Journal of Enology and Viticulture 2013; 34(1) : 54-67.
AUDOUIN M, PREISER R, NIENABER S, DOWNSBOROUGH L, LANZ J, MAVENGAHAMA S. Exploring the implications of critical complexity for the study of social-ecological systems. Ecology and Society 2013; 18(3) : Art.#12.
BLOEM LM, STORBECK K-H, SCHLOMS L, SWART AC. 11?-Hydroxyandrostenedione returns to the steroid arena: Biosynthesis, metabolism and function. Molecules 2013; 18 : 13228-13244.
BURGER H-M, SHEPHARD GS, LOUW W, RHEEDER JP, GELDERBLOM WCA. The mycotoxin distribution in maize milling fractions under experimental conditions. International Journal of Food Microbiology 2013; 165 : 57-64.
CILLIERS FP, BIGGS HC, BLIGNAUT S, CHOLES AG, HOFMEYR J-HS, JEWITT GPW, ROUX DJ. Complexity, modeling, and natural resource management. Ecology and Society 2013; 18(3) : 1.
DE VILLIERS M, MACUAMULE C, SPRY C, HYUN Y-M, STRAUSS E, SALIBA KJ. Structural modification of pantothenamides counteracts degradation by pantetheinase and improves antiplasmodial activity. ACS Medicinal Chemistry Letters 2013; 4 : 784-789.
DE VILLIERS MJ, PIRIE MD, HUGHES M, M?LLER M, EDWARDS TJ, BELLSTEDT DU. An approach to identify putative hybrids in the 'coalescent stochasticity zone', as exemplified in the African plant genus Streptocarpus (Gesneriaceae). New Phytologist 2013; 198 : 284-300.
DU PLESSIS DM, BOTES M, DICKS LMT, CLOETE TE. Immobilization of commercial hydrolytic enzymes on poly (acrylonitrile) nanofibers for anti-biofilm activity. Journal of Chemical Technology and Biotechnology 2013; 88(4) : 585-593.
GEENEN S, DU PREEZ F, SNOEP JL, FOSTER AJ, SARDA S, KENNA JG, WILSON ID, WESTERHOFF H V. Glutathione metabolism modeling: A mechanism for liver drug-robustness and a new biomarker strategy. Biochimica et Biophysica Acta-General Subjects 2013; 1830 : 4943-4959.
GOOSEN P, SWART AC, STORBECK K-H, SWART P. Allosteric interaction between 3?-hydroxysteroid dehydrogenase/? 5-?4 isomerase and cytochrome b5 influences cofactor binding. Faseb Journal 2013; 27 : 322-332.
HOFMEYR J-HS, GQWAKA OPC, ROHWER JM. A generic rate equation for catalysed, template-directed polymerisation. Febs Letters 2013; 587 : 2868-2875.
HOUGH D, CLOETE SWP, STORBECK K-H, SWART AC, SWART P. Cortisol production in sheep is influenced by the functional expression of two cytochrome P450 17á-hydroxylase/17,20-lyase (CYP17) isoforms. Journal of Animal Science 2013; 91 : 1193-1206.
KALILI KM, VESTNER J, STANDER MA, DE VILLIERS AJ. Toward unraveling grape tannin composition: Application of online hydrophilic interaction chromatography x reversed-phase liquid chromatography-time-of-flight mass spectrometry for grape seed analysis. Analytical Chemistry 2013; 85 : 9107-9115.
KOURIL T, ESSER D, KORT J, WESTERHOFF H V, SIEBERS B, SNOEP JL. Intermediate instability at high temperature leads to low pathway efficiency for an in vitro reconstituted system of gluconeogenesis in Sulfolobus solfataricus. Febs Journal 2013; 280 : 4666-4680.
KRITZINGER EC, STANDER MA, DU TOIT WJ. Assessment of glutathione levels in model solution and grape ferments supplemented with glutathione-enriched inactive dry yeast preparations using a novel UPLC-MS/MS method. Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment 2013; 30(1) : 80-92.
LOMBARD MJ, STEYN N, BURGER H-M, CHARLTON K, SENEKAL M. A food photograph series for identifying portion sizes of culturally specific dishes in rural areas with high incidence of oesophageal cancer. Nutrients 2013; 5 : 3118-3130.
LOUW A, JOUBERT E, VISSER K. Phytoestrogenic potential of Cyclopia extracts and polyphenols. Planta Medica 2013; 79 : 580-590.
MOLERO C, PETR?NYI K, GONZ?LEZ A, CARMONA M, GELIS S, ABRIE JA, STRAUSS E, RAMOS J, DOMBRADI V, HIDALGO E, ARI?O J. The Schizosaccharomyces pombe fusion gene hal3 encodes three distinct activities. Molecular Microbiology 2013; 90(2) : 367-382.
MUNYUKI G, JACKSON GE, VENTER GA, K?V?R KE, SZIL?GYI L, RAUTENBACH M, SPATHELF B, BHATTACHARYA B, VAN DER SPOEL D. ??Sheet structures and dimer models of the two major tyrocidines, antimicrobial peptides from Bacillus aneurinolyticus. Biochemistry 2013; 52 : 7798-7806.
NAIDOO VB, RAUTENBACH M. Self-assembling organo-peptide bolaphiles with KLK tripeptide head groups display selective antibacterial activity. Journal of Peptide Science 2013; 19 : 784-791.
PALM DC, ROHWER JM, HOFMEYR J-HS. Regulation of glycogen synthase from mammalian skeletal muscle – a unifying view of allosteric and covalent regulation. Febs Journal 2013; 280 : 2-27.
PILLAY CS, HOFMEYR J-HS, MASHAMAITE LN, ROHWER JM. From top-down to bottom-up: Computational modeling approaches for cellular redoxin networks. Antioxidants & Redox Signaling 2013; 18(16) : 2075-2086.
QUINTON-TULLOCH MJ, BRUGGEMAN FJ, SNOEP JL, WESTERHOFF H V. Trade-off of dynamic fragility but not of robustness in metabolic pathways in silico. Febs Journal 2013; 280 : 160-173.
ROBERTSON S, HAPGOOD JP, LOUW A. Glucocorticoid receptor concentration and the ability to dimerize influence nuclear translocation and distribution. Steroids 2013; 78 : 182-194.
ROBERTSON S, ROHWER JM, HAPGOOD JP, LOUW A. Impact of glucocorticoid receptor density on ligand-independent dimerization, cooperative ligand-binding and basal priming of transactivation: A cell culture model. PLoS ONE 2013; 8(5) : e64831.
ROGERS KH, LUTON R, BIGGS H, BIGGS R, BLIGNAUT S, CHOLES AG, PALMER CG, TANGWE P. Fostering Complexity Thinking in Action Research for Change in Social–Ecological Systems. Ecology and Society 2013; 18(2) : Art.#31.
SHEPHARD GS, KIMANYA ME, KPODO KA, GNONLONFIN GJB, GELDERBLOM WCA. The risk management dilemma for fumonisin mycotoxins. Food Control 2013; 34 : 596-600.
SMIT AY, DU TOIT WJ, STANDER MA, DU TOIT M. Evaluating the influence of maceration practices on biogenic amine formation in wine. LWT-Food Science and Technology 2013; 53 : 297-307.
SPRY C, MACUAMULE C, LIN Z, VIRGA KG, LEE RE, STRAUSS E, SALIBA KJ. Pantothenamides are potent, on-target inhibitors of Plasmodium falciparum growth when serum pantetheinase is inactivated. PLoS ONE 2013; 8(2) : e54974.
STANDER MA, K?HN W, HITEN NF. Survey of South African fruit juices using a fast screening HILIC-MS method. Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment 2013; 30(9) : 1473-1484.
STORBECK K-H, BLOEM LM, AFRICANDER D, SCHLOMS L, SWART P, SWART AC. 11?-Hydroxydihydrotestosterone and 11-ketodihydrotestosterone, novel C19 steroids with androgenic activity: A putative role in castration resistant prostate cancer?. Molecular and Cellular Endocrinology 2013; 377 : 135-146.
STORBECK K-H, SWART AC, GOOSEN P, SWART P. Cytochrome b5: Novel roles in steroidogenesis. Molecular and Cellular Endocrinology 2013; 371 : 87-99.
SWART AC, BLOEM LM, SCHLOMS L, SWART P, AFRICANDER D, STORBECK K-H. 11?-Hydroxyandrostenedione is metabolised to novel androgenic C19 steroids in the prostate. International Journal of Molecular Medicine 2013; 32(1) : S21.
SWART AC, SCHLOMS L, STORBECK K-H, BLOEM LM, DU TOIT T, QUANSON JL, RAINEY WE, SWART P. 11?-Hydroxyandrostenedione, the product of androstenedione metabolism in the adrenal, is metabolized in LNCaP cells by 5á-reductase yielding 11?-hydroxy-5á-androstanedione. Journal of Steroid Biochemistry and Molecular Biology 2013; 138 : 132-142.
SWART P, SWART AC, LOUW A, DE BOSSCHER K, HAEGEMAN G, BUDONOVA I. Anti-inflammatory and anti-cancer activities of compound A: A synthetic compound derived from a natural product. International Journal of Molecular Medicine 2013; 32(1) : S21.
VAN DER WESTHUIZEN L, SHEPHARD GS, GELDERBLOM WCA, TORRES O, RILEY RT. Fumonisin biomarkers in maize eaters and implications for human disease. World Mycotoxin Journal 2013; 6(3) : 223-232.
VISSER K, MORTIMER M, LOUW A. Cyclopia extracts act as ER?á antagonists and ER?? agonists, in vitro and in vivo. PLoS ONE 2013; 8(11) : e79223.
VOSLOO JA, STANDER MA, LEUSSA AN-N, SPATHELF B, RAUTENBACH M. Manipulation of the tyrothricin production profile of Bacillus aneurinolyticus. Microbiology 2013; 159 : 2200-2211.
WILLEMSE CM, STANDER MA, DE VILLIERS AJ. Hydrophilic interaction chromatographic analysis of anthocyanins.
Journal of Chromatography A 2013; 1319 : 127-140.??
Journal Articles (non-subsidised)
HOUGH D, SWART P, CLOETE SWP. Exploration of the hypothalamic-pituitary-adrenal axis to improve animal welfare by means of genetic selection: Lessons from the South African Merino. Animals 2013; 3 : 442-474.
SCHEINPFLUG K, NIKOLENKO H, KOMAROV IV, RAUTENBACH M, DATHE M. What goes around comes around-A comparative study of the influence of chemical modifications on the antimicrobial properties of small cyclic peptides. Pharmaceuticals 2013; 6 : 1130-1144.?
Proceedings International
HOUGH D, SWART P, CLOETE JJE, CLOETE SWP. Searching for SNPs that affect sheep robustness: CYP17 SNP affects behavioural responses to psychological stress. Proceeding of the Association for the Advancement of Animal Breeding and Genetics, Massey 中国体育彩票, Napier, New Zealand, AAABG Distribution Service 2013: 503-506.?
Chapters in Books
SNOEP JL. Databases for Kinetic Models. In
Encyclopedia of Systems Biology, Springer, New York, USA, 2013: 537-544.?
Doctorals completed?
BURGER H-M. Altered lipid metabolism as a possible mechanism in fumonisin-induced hepatocarcinogenesis in rats and investigations into risk assessment in humans. PhD(Bioch), 2013. 353 pp. Promotor: Gelderblom WCA. Medepromotor: Swart P.
EICHER JJ. Understanding glycolysis in Escherichia coli: A systems approach using nuclear magnetic resonance spectroscopy. PhD(Bioch), 2013. 197 pp. Promotor: Rohwer JM. Medepromotor: Snoep JL.
LOUW R. A study of the molecular mechanism of progestin-induced regulation of IL-12 and IL-10 and implications for HIV pathogenesis. PhD(Bioch), 2013. 215 pp. Promotor: Africander D. Medepromotor: Hapgood JP.
MAGCWEBEBA T. Chemopreventive properties of South African herbal teas, Rooibos, (Aspalathus linearis) and Honeybush (Cyclopia spp): Mechanism against skin carcinogenesis. PhD(Bioch), 2013. 269 pp. Promotor: Gelderblom WCA. Medepromotor: Joubert E, Swart P.
PALM DC. The regulatory design of glycogen metabolism in mammalian skeletal muscle. PhD(Bioch), 2013. 285 pp. Promotor: Hofmeyr J-HS. Medepromotor: Rohwer JM.
PENKLER G. A kinetic model of glucose catabolism in Plasmodium falciparum. PhD(Bioch), 2013. 196 pp. Promotor: Snoep JL, Westerhoff H V. Medepromotor: Rautenbach M.?
Masters completed
ADRIAANSE C. An investigation into the complex formation of membrane bound cytochrome b5 isolated from ovine liver microsomes. MSc Biochemie, 2013. 103 pp. Studieleier: Swart P. Medestudieleier: Rautenbach M.
CHRISTENSEN CD. Applications of generalised supply-demand analysis. MSc Biochemie, 2013. 134 pp. Studieleier: Rohwer JM. Medestudieleier: Hofmeyr J-HS.
KLOPPER SL. Cloning and functional characterization of thiol disulfide interchange system proteins from Staphylococcus aureus. MSc Biochemie, 2013. 90 pp. Studieleier: Strauss E.
MOCKE L. Kinetic modelling of wine fermentations: Why does yeast prefer glucose to fructose?. MSc Biochemie, 2013. 78 pp. Studieleier: Snoep JL.
ROOS WG. An investigation of prevalence and the detection and race identification of South African potato viruses. MSc Biochemie, 2013. 155 pp. Studieleier: Bellstedt DU.
STRYDOM M. The ostrich mycoplasma Ms02 Partial genome assembly, bioinformatic analysis and the development of three DNA vaccines. MSc Biochemie, 2013. 150 pp. Studieleier: Botes A. Medestudieleier: Bellstedt DU.
VAN TONDER A. Development of an oral vaccine against the ostrich-specific mycoplasma, Mycoplasma struthionis. MSc Biochemie, 2013. 220 pp. Studieleier: Bellstedt DU. Medestudieleier: Botes A.
VAN ZYL J. The quantification of metabolic regulation. MSc Biochemie, 2013. 124 pp. Studieleier: Hofmeyr J-HS. Medestudieleier: Rohwer JM.?
?Publications in 2012
?Journal Articles (subsidised)?
ABRIE JA, GONZALEZ A, STRAUSS E, ARINO J. Functional mapping of the disparate activities of the yeast moonlighting protein Hal3. Biochemical Journal 2012; 442 : 357-368.
ALBRECHT CF, STANDER MA, GROBBELAAR MC, COLLING J, KOSSMANN J, HILLS PN, MAKUNGA NP. LC-MS-based metabolomics assists with quality assessment and traceability of wild and cultivated plants ofSutherlandia frutescens (Fabaceae). South African Journal of Botany 2012; 82 : 33-45.
J. Reuteran and levan as carbohydrate sinks in transgenic sugarcane. Planta 2012; 236 : 1803-1815.
BAUER R, BASSON CE, BEKKER J, EGBICHI IM, ROHWER JM, UYS L, VAN WYK JH, KOSSMANN J. Reuteran and levan as carbohydrate sinks in transgenic sugarcane. Planta 2012; 236(6) : 1803-1815.
BELLSTEDT DU, GALLEY C, PIRIE MD, LINDER HP. The Migration of the Palaeotropical Arid Flora: Zygophylloideae as an Example. Systematic Botany 2012; 37(4) : 951-959.
CUEVAS JM, DELAUNAY A, VISSER JC, BELLSTEDT DU, JACQUOT E, ELENA SF. Phylogeography and Molecular Evolution of Potato virus Y. PLoS ONE 2012; 7(5) : e37853.
DE BEER D, SCHULZE AE, JOUBERT E, DE VILLIERS AJ, MALHERBE CJ, STANDER MA. Food ingredient extracts of Cyclopia subternata (Honeybush): Variation in phenolic composition and antioxidant capacity. Molecules2012; 17 : 14602-14624.
DU PLESSIS DM, BOTES M, DICKS LMT, CLOETE TE. Immobilization of commercial hydrolytic enzymes on poly (acrylonitrile) nanofibers for anti-biofilm activity . Journal of Chemical Technology and Biotechnology 2013; 88(4) : 585-593.
DU PREEZ F, VAN NIEKERK DD, KOOI BW, ROHWER JM, SNOEP JL. From steady-state to synchronized yeast glycolytic oscillations I: model construction. Febs Journal 2012; 279 : 2810-2822.
DU PREEZ F, VAN NIEKERK DD, SNOEP JL. From steady-state to synchorinized yeast glycolytic oscillations II: model validation. Febs Journal 2012; 279 : 2823-2836.
GEENEN S, DU PREEZ F, REED M, NIJHOUT HF, KENNA JG, WILSON ID, WESTERHOFF HW, SNOEP JL. A mathematical modelling approach to assessing the reliability of biomarkers of glutathione metabolism. European Journal of Pharmaceutical Sciences 2012; 46 : 233-243.
GELDERBLOM WCA, MARASAS WFO. Controversies in fumonisin mycotoxicology and risk assessment. Human & Experimental Toxicology 2012; 3 : 215-235.
GUSTAVSSON A-K, VAN NIEKERK DD, ADIELS CB, DU PREEZ F, GOKS?R M, SNOEP JL. Sustained glycolytic oscillations in individual isolated yeast cells. Febs Journal 2012; 279 : 2837-2847.
KADEREIT G, ACKERLY D, PIRIE MD. A broader model for C4 photosynthesis evolution in plants inferred from the goosefoot family (Chenopodiaceae s.s.). Proceedings of the Royal Society B-Biological Sciences 2012; 279 : 3304-3311.
KISSLING J. Taxonomy of Exochaenium and Lagenias: Two Resurrected Genera of Tribe Exaceae (Gentianaceae).Systematic Botany 2012; 37(1) : 238-253.
KOLODKIN A, BOOGERD FC, PLANT N, BRUGGEMAN F, GONCHARUK V, LUNSHOF J, MORENO-SANCHEZ R, YILMAZ N, BAKKER BM, SNOEP JL, BALLING R, WESTERHOFF HW. Emergence of the silicon human and network targeting drugs. European Journal of Pharmaceutical Sciences 2012; 46 : 190-197.
LAMPRECHT DA, MUNERI NO, EASTWOOD H , NAIDOO K, STRAUSS E, JARDINE A. An enzyme-initiated Smiles rearrangement enables the development of an assay of MshB, the GlcNAc-Ins deacetylase of mycothil biosynthesis. Organic & Biomolecular Chemistry 2012; 10 : 5278.
LONG HS, STANDER MA, VAN WYK B.-E.. Notes on the occurrence and significance of triterpenoids (asiaticoside and related compounds) and caffeoylquinic acids in Centella species. South African Journal of Botany 2012; 82 : 53-59.
MARASAS WFO, GELDERBLOM WCA, SHEPHARD GS, VISMER HF. Mycotoxicological research in South Africa 1910-2011. World Mycotoxin Journal 2012; 5(1) : 89-102.
NAIDOO VM, RAUTENBACH M. Bidirectional solid phase synthesis of a model oligoglycine bolaamphiphile and purification by rapid self-assembly. Journal of Peptide Science 2012; 18 : 317-325.
PIRIE MD, HUMPHREYS AM, ANTONELLI A, GALLEY C, LINDER HP. Model uncertainty in ancestral are reconstruction: A parsimonious solution?. Taxon 2012; 61(3) : 652-664.
RAUTENBACH M, EY?GH?-BIKONG HA, VLOK NM, STANDER MA, DE BEER A. Direct surfactin-gramicidin S antagonism supports detoxification in mixed producer cultures of Bacillus subtilis and Aneurinibacillus migulanux.Microbiology 2012; 158 : 3072-3082.
ROHWER JM. Kinetic modelling of plant metabolic pathways. Journal of Experimental Botany 2012; 63(6) : 2275-2292.
SCHLOMS L, STORBECK K-H, SWART P, GELDERBLOM WCA, SWART AC. The influence of Aspalathus linearis(Rooibos) and dihydrochalcones on adrenal steroidogenesis: Quantification of steroid intermediates and end products in H295R cells. Journal of Steroid Biochemistry and Molecular Biology 2012; 128 : 128-138.
M. On modifying the Arrhenius equation to compensate for temperature changes for reactions within biological systems. Water SA 2012; 38(1) : 149-151.
STORBECK K-H, SWART AC, LOMBARD N, ADRIAANSE C, SWART P. Cytochrome b5 forms homomeric complexies in living cells. Journal of Steroid Biochemistry and Molecular Biology 2012; 132 : 311-321.
THOMPSON GD, BELLSTEDT DU, BYRNE M, MILLAR MA, RICHARDSON DM, WILSON JRU, LE ROUX J. Cultivation shapes genetic novelty in a globally important invader. Molecular Ecology 2012; 21 : 3187-3199.
TROSKIE AM, VLOK NM, RAUTENBACH M. A novel 96-well gel-based assay for determining antifungal activity against filamentous fungi. Journal of Microbiological Methods 2012; 91 : 551-558.
VAN DER MERWE JD, JOUBERT E, MANLEY M, DE BEER D, MALHERBE CJ, GELDERBLOM WCA. Mangiferin glucuronidation: Important hepatic modulation of antioxidant activity. Food and Chemical Toxicology 2012; 50 : 808-815.
VAN DER WESTHUIZEN R, HAMMONS JC, MEIER JL, DAHESH S, MOOLMAN WJA, PELLY SC, NIZET V, BURKART MD, STRAUSS E. The antibiotic CJ-15,801 is an antimetabolite that hijacks and then inhibits CoA biosynthesis. Chemistry & Biology 2012; 19 : 559-571.
VISSER JC, BELLSTEDT DU, PIRIE MD. The Recent Recombinant Evolution of a Major Crop Pathogen, Potato virus Y. PLoS ONE 2012; 7(11) :e50631.
WALLACE BD, EDWARDS JS, WALLEN JR, MOOLMAN WJA, VAN DER WESTHUYZEN R, STRAUSS E, REDINBO MR, CLAIBORNE A. Turnover-Dependent Covalent Inactivation of Staphylococcus aureus Coenzyme A-Disulfide Reductase by Coenzyme A-Mimetics: Mechanistic and Structural Insights. Biochemistry 2012; 51 : 7699-7711.
Journal Articles (non-subsidised)
EICHER JJ, SNOEP JL, ROHWER JM. Determining Enzyme Kinetics for Systems Biology with Nuclear Magnetic Resonance Spectroscopy. Metabolites 2012; 2 : 818-843.
MAGCWEBEBA T, RIEDEL S, SWANEVELDER S, BOUIC P, SWART P, GELDERBLOM WCA. Interleukin-1alpha Induction in Human Keratinocytes (HaCaT): An In Vitro Model for Chemoprevention in Skin. Journal of Skin Cancer2012; 2012 : 1-10.
Proceedings National
THOMPSON GD, BELLSTEDT DU, LE ROUX JJ, RICHARDSON DM, WILSON JRU. Acacia saligna invasions: Genetics and species distribution modeling. South African Association of Botanists 38th Conference, 中国体育彩票 of Pretoria, Pretoria, South Africa, South African Journal of Botany 2012: 215-216.
Books
PITT JI, WILD CP, BAAN RA, GELDERBLOM WCA, MILLER JD, RILEY RT, WU F. Improving Public Health Through Mycotoxin Control. The International Agency for Research on Cancer, Lyon Cedex, France 2012:151 pp.
?Chapters in Books
MARASAS WFO, SHEPHARD GS, VISMER HF, ALBERTS JF, VAN DER WESTHUIZEN L, GELDERBLOM WCA. History and Relevance of Fumonisins. In World Nutrition Forum, Anytine Publishing, Leicestershire, United Kingdom, 2012: 213-240.?
???Doctorals completed?
ABRIE JA. Investigation of the structural and fundtional diversity of phoshopantothenoylcysteine decarboxylases. PhD(Bioch), 2012. 164 pp. Promotor: Strauss E.
GOOSEN P. The influence of 3?HSD on adrenal steroidogenesis and the facors which influecne its activity. PhD(Bioch), 2012. 135 pp. Promotor: Swart P. Medepromotor: Swart AC.
HOUGH D. Comparison of two CYP17 isoforms: impllications for cortisol production in the South African Merino. PhD(Bioch), 2012. 154 pp. Promotor: Swart P. Medepromotor: Cloete SWP.
MFA MEZUI A. The effect of ultraviolet-C treatment on the biochemical composition of beer. PhD(Bioch), 2012. 186 pp. Promotor: Swart P. Medepromotor: Rautenbach M.
VISSER JC. A study of the strain evolution and recombination of South African isolates of Potato virus Y. PhD(Bioch), 2012. 180 pp. Promotor: Bellstedt DU.
Masters completed
BARNARD A. The optimization of the extraction and purification of horseradish peroxidase from horseradish roots. MSc Biochemie, 2012. 132 pp. Studieleier: Swart P. Medestudieleier: Graz M.
BRANDT S. Preliminary investigations into the use of DNA vaccines to elicit protective immune responses against the ostrich mycoplasma MS01. MSc Biochemie, 2012. 121 pp. Studieleier: Bellstedt DU. Medestudieleier: Botes A.
DE WET T. Laboratory optimization of a protease extraction and purification process from bovine pancreas in preparation for industrial scale up. MSc Biochemie, 2012. 198 pp. Studieleier: Swart P. Medestudieleier: Graz M.
DODD JA. Protein immobilisation on magnetic nanoparticles for the production of lactose-free milk. MSc Biochemie, 2012. 112 pp. Studieleier: Swart P. Medestudieleier: Klumperman B.
GOOSEN R. Quantitative in vitro analysis of Coenzyme A salvage pathway metabolites in Escherichia coli: Development and Application. MSc Biochemie, 2012. 91 pp. Studieleier: Strauss E. Medestudieleier: Snoep JL.
MAKOWA H. The relationship between the insecticide dichlorodiphenyl-trichloroethane and chloroquine in Plasmodium falciparum resistance. MSc Biochemie, 2012. 137 pp. Studieleier: Rautenbach M.
MUNERI NO. Biocatalytic preparation and characterization of alternative substrate of MshB, a mycothiol pathway enzyme. MSc Biochemie, 2012. 72 pp. Studieleier: Strauss E.
NOETH D. Regulation of chemokine gene expression by synthetic progestins in a human vaginal epithelial cell line. MSc Biochemie, 2012. 114 pp. Studieleier: Africander D. Medestudieleier: Hapgood JP.
PRETORIUS C. Covalent immobilisation of alpha-Galactosidase from Escherichia coli to commercially available magnetic nanoparticles for the removal of lactose from milk. MSc Biochemie, 2012. 130 pp. Studieleier: Swart P. Medestudieleier: Storbeck K-?H
?
Publications in 2011
Journal Articles (subsidised)
AERTS R, THIJS KW, LEHOUCK V, BEENTJE H, BYTEBIER BLG, MATTHYSEN E, GULINCK H, LENS L, MUYS B. Woody plant communities of isolated Afromontane cloud forests in Taita Hills, Kenya. Plant Ecology 2011; 212 : 639-649.
AFRICANDER D, LOUW R, VERHOOG N, NOETH D, HAPGOOD JP. Differential regulation of endogenous pro-inflammatory cytokine genes by medroxyprogesterone acetate and norethisterone acetate in cell lines of the female genital tract. Contraception 2011; 84(4) : 423-435.
AFRICANDER D, VERHOOG N, HAPGOOD JP. Molecular mechanisms of steroid receptor-mediated actions by synthetic progestins used in HRT and contraception. Steroids 2011; 76 : 636-652.
BYTEBIER BLG, ANTONELLI A, BELLSTEDT DU, LINDER HP. Estimating the age of fire in the Cape flora of South Africa from an orchid phylogeny. Proceedings of the Royal Society B-Biological Sciences 2011; 278 : 188-195.
CLOETE WJ, ADRIAANSE C, SWART P, KLUMPERMAN B. Facile immobilization of enzymes on electrospun poly(styrene-alt-maleic anhydride) nanofibres. Polymer Chemistry 2011; 2 : 1479-1481.
COUVREUR TLP, PIRIE MD, CHATROU LW, SAUNDERS RMK, SU YCF, RICHARDSON JE, ERKENS RHJ. Early evolutionary history of the flowering plant family Annonaceae: steady diversification the boreotropical geodispersal. Journal of Biogeography 2011; 38 : 664-680.
DAVIES TJ, SMITH GF, BELLSTEDT DU, BOATWRIGHT JS, BYTEBIER BLG, COWLING RM, FOREST F, HARMON LJ, MUASYA AM, SCHRIRE B, STEENKAMP Y, VAN DER BANK M, SAVOLAINEN V. Extinction Risk and Diversification Are Linked in a Plant Biodiversity Hotspot. Plos Biology 2011; 9(5) : e1000620.
DEKKER S, CILLIERS P, HOFMEYR J-HS. The complexity of failure: Implications of complexity theory for safety investigations. Safety Science 2011; 49 : 939-945.
GOOSEN P, STORBECK K-H, SWART AC, CONRADIE R, SWART P. Cytochrome b5 augments 3?-hydroxysteroid dehydrogenase/?5-?4 isomerase activity. Journal of Steroid Biochemistry and Molecular Biology 2011; 127 : 238-247.
HADLEY K, LOUW A, HAPGOOD JP. Differential nuclear localisation and promoter occupancy play a role in glucocorticoid receptor ligand-specific transcriptional responses. Steroids 2011; 76 : 1176-1184.
HOFMEYR J-HS, ROHWER JM. Supply-Demand Analysis: A Framework for Exploring the Regulatory Design of Metabolism. Methods in Enzymology 2011; 500(Chapter 25) : 533-554.
LARSSON C, SNOEP JL, NORBECK J, ALBERS E. Flux balance analysis for ethylene formation in genetically engineered Saccharomyces cerevisiae. IET Systems Biology 2011; 5(4) : 245-251.
MFA MEZUI A, SWART P. Effect of UV-C Disinfection of Beer – Sensory Analysis and Consumer Ranking. Journal of the Institute of Brewing 2010; 116(4) : 348-353.
OBERLANDER KC, DREYER LL, BELLSTEDT DU. Molecular phylogenetics and origins of southern African Oxalis. Taxon 2011; 60(6) : 1667-1677.
PILLAY CS, HOFMEYR J-HS, ROHWER JM. The logic of kinetic regulation in the thioredoxin system. BMC SYSTEMS BIOLOGY 2011; 5 : 15.
PIRIE MD, OLIVER EGH, BELLSTEDT DU. A densely sampled ITS phylogeny of the Cape flagship genus Erica L. suggests numerous shifts in floral macro-morphology . Molecular Phylogenetics and Evolution 2011; 61 : 593–601.
SHERIDAN CM, GLASSER D, HILDEBRANDT D, PETERSEN J, ROHWER JM. An Annual and Seasonal
Characterisation of Winery Effluent in South Africa. South African Journal of Enology and Viticulture 2011; 32(1) : 1-8.
SISSING L, MARNEWICK JL, DE KOCK M, SWANEVELDER S, JOUBERT E, GELDERBLOM WCA. Modulating effects of rooibos and honeybush herbal teas on the development of esophageal papillomas in rats. Nutrition and Cancer-An International Journal 2011; 63(4) : 600-610.
VAN DER WESTHUIZEN L, SHEPHARD GS, BURGER H.-M., RHEEDER JP, GELDERBLOM WCA, WILD CP, GONG YY. Fumonisin B1 as a Urinary Biomarker of Exposure in a Maize Invervention Study Among South African Subsistence Farmers. Cancer Epidemiology Biomarkers & Prevention 2011; 20 : 483-489.
VAN DER WESTHUIZEN L, SHEPHARD GS, RHEEDER JP, BURGER H.-M., GELDERBLOM WCA, WILD CP, GONG YY. Optimising sorting and washing of home-grown maize to reduce fumonisin contamination under laboratory-controlled conditions. Food Control 2011; 22 : 396-400.
WALTEMATH D, ADAMS R, BEARD DA, BERGMAN FT, BHALLA U S, BRITTEN R, CHELLIAH V, COOLING MT, COOPER J, CRAMPIN E J , GARNY A, HOOPS S, HUCKA M, HUNTER P, KLIPP E, LAIBE C, MILLER AK, MORARU I, NICKERSON D, NIELSEN P, NIKOLSKI M, SAHLE S, SAURO H, SCHMIDT H,. Minimum Information 中国体育彩票 a Simulation Experiment (MIASE). Plos Computational Biology 2011; 7(4) : e1001122.
WALTEMATH D, ADAMS R, BERGMANN FT, HUCKA M, KOLPAKOV F, MILLER AK, MORARU I, NICKERSON D, SAHLE S, SNOEP JL, LE NOV?RE N . Reproducible computational biology experiments with SED-ML – The Simulation Experiment Description Markup Language. BMC SYSTEMS BIOLOGY 2011; 5(198) : 175-0505: 1-10.
WARREN BH, BAKKER FT, BELLSTEDT DU, BYTEBIER BLG, CLABEN-BOCKHOFF R, DREYER LL, EDWARDS D, FOREST F, GALLEY C, HARDY CR, LINDER HP, MUASYA AM, MUMMENHOFF K, OBERLANDER KC, QUINT M, RICHARDSON JE, SAVOLAINEN V, VAN DER NIET T , SCHRIRE B, VAN DER NIET T , . Consistent phenological shifts in the making of a biodiversity hotspot: the Cape flora. BMC Evolutionary Biology 2011; 11(39) : 1-11.
WOLSTENCROFT K, OWEN S, DU PREEZ F, KREBS O, MUELLER W, GOBLE C, SNOEP JL. The Seek: A Platform for Sharing Data and Models in Systems Biology. Methods in Enzymology 2011; 500(Chapter 29) : 629-655.
WOLSTENCROFT K, OWEN S, HORRIDGE M, KREBS O, MUELLER W, SNOEP JL, DU PREEZ F, GOBLE C. RightField: embedding ontology annotation in spreadsheets. Bioinformatics 2011; 27(14) : 2021-2022.
Journal Articles (non-subsidised)
SWAT M, KIELBASA SM, POLAK S, OLIVIER BG, BRUGGEMAN F, TULLOCH MQ, SNOEP JL, VERHOEVEN AJ, WESTERHOFF HW. What it takes to understand and cure a living system: computational systems biology and a systems biology-driven pharmacokinetics-pharmacodynamics platform. Interface Focus 2011; 1 : 16-23.
Chapters in Books
HUGENHOLTZ J, HOLS P, SNOEP JL. Metabolic engineering of lactic acid bacteria. In Thirty years of research on lactic acid bacteria. (ISBN 978-90-817671-0-1), 24 Media Labs, Rotterdam, Netherlands, 2011: 113-133.
???Doctorals completed?
EY?GH?-BIKONG HA. Role of surfactin from Bacillus subtilis in protection against antimicrobial peptides produced by Bacillus species. PhD(Bioch), 2011. 239 pp. Promotor: Rautenbach M.
ROBERTSON S. Receptor concentration affects glucocorticoid action. PhD(Bioch), 2011. 200 pp. Promotor: Louw A. Medepromotor: Hapgood JP.?
Masters completed?
CROUS C. Supply-demand analysis of anaerobic free-energy metabolism in Zymomonas mobilis. MSc Biochemie, 2011. 85 pp. Studieleier: Rohwer JM. Medestudieleier: Snoep JL.
DU PLESSIS DM. Fabrication and characterization of anti-microbial and biofouling resistant nanofibers with silver nanoparticles and immobilized enzymes for application in water filtration. MSc Biochemie, 2011. 94 pp. Studieleier: Cloete TE. Medestudieleier: Dicks LTM, Swart P.
GQWAKA OPC. A generic rate equotion for catalysed, template-directed polymerisation and its use in computational systems biology. MSc Biochemie, 2011. 111 pp. Studieleier: Hofmeyr J-HS. Medestudieleier: Rohwer JM.
JANA CE. Synthesis and evaluation of pantothenic acid analogues as potential inhibitors of malaria parasites and bacteria. MSc Biochemie, 2011. 99 pp. Studieleier: Strauss E.
JORDAAN S. Supply-demand analysis of energy metabolism in Lactococcus Lactis under anerobic conditions. MSc Biochemie, 2011. 83 pp. Studieleier: Rohwer JM. Medestudieleier: Snoep JL.?
Publications in 2010
Journal Articles (subsidised)
APWEILER R, ARMSTRONG R, BAIROCH A, CORNISH-BOWDEN A, HALLING PJ, HOFMEYR J-HS, KETTNER C, LEYH TS, ROHWER JM, SCHOMBURG D, STEINBECK C, TIPTON K. A large-scale protein-function database.Nature Chemical Biology 2010; 6 : 785.
AVENANT C, RONACHER K, STUBSRUD EF, LOUW A, HAPGOOD JP. Role of ligand-dependent GR phosphorylation and half-life in determination of ligand-specific transcriptional activity. Molecular and Cellular Endocrinology 2010; 327 : 72-88.
BELLSTEDT DU, PIRIE MD, VISSER C, DE VILLIERS M, GEHRKE B. A Rapid and Inexpensive Method for the Direct PCR Amplification of DNA from Plants. American Journal of Botany 2010; 97(7) : 65-68.
BURGER H-M, LOMBARD MJ, SHEPHARD GS, RHEEDER JR, VAN DER WESTHUIZEN L, GELDERBLOM WCA. Dietary fumonisin exposure in a rural population of South Africa. Food and Chemical Toxicology 2010; 48 : 2103-2108.
CONRADIE R, BRUGGEMAN F, CILIBERTO A, CSIKASZ-NAGY A, NOVAK B, WESTERHOFF HW, SNOEP JL. Restriction point control of the mammalian cell cycle via the cyclin E/Cdk2:p27 complex. Febs Journal 2010; 277 : 357-367.
DE VILLIERS J, KOEKEMOER L, STRAUSS E. 3-Fluoroaspartate and Pyruvoyl-Dependant Aspartate Decarboxylase: Exploiting the Unique Characteristics of Fluorine To Probe Reactivity and Binding. Chemistry-A European Journal 2010; 16 : 10030-10041.
GOOSEN P, SWART AC, STORBECK K-H, SWART P. Hypocortisolism in the South African Angora goat: The role of 3?HSD. Molecular and Cellular Endocrinology 2010; 315 : 182-187.
HILPERT K, MCLEOD B, YU J, ELLIOTT MR, RAUTENBACH M, RUDEN S, BURCK J, MUHLE-GOLL C, ULRICH AS, KELLER S, HANCOCK REW. Short Cationic Antimicrobial Peptides Interact with ATP. Antimicrobial Agents and Chemotherapy 2010; 54(10) : 4480-4483.
JOOSTE AEC, MAREE HJ, BELLSTEDT DU, GOSZCZYNSKI DE, PIETERSEN GJ, BURGER JT . Three genetic grapevine leafroll-associated virus 3 variants identified from South African vineyards show high variability in their 5′UTR. Archives of Virology 2010; 155 : 1997-2006.
KOLODKIN A, BRUGGEMAN F, PLANT N, MON? MJ , BAKKER BM, CAMPBELL MJ, VAN LEEUWEN JPTM, CARLBERG C, SNOEP JL, WESTERHOFF HW. Design principles of nuclear receptor signaling: how complex networking improves signal transduction. Molecular Systems Biology 2010; 6(446) : 1-14.
LI C, DONIZELLI M, RODRIGUEZ N, DHARURI H, ENDLER L, CHELLIAH V, LI L, HE E, HENRY A, STEFAN MI, SNOEP JL, HUCKA M, LE NOV?RE N , LAIBE C. BioModels Databse: An enhanced, curated and annotated resource for published quantitative kinetic models. BMC SYSTEMS BIOLOGY 2010; 4(92) : 1-2.
ROBERTSON S, ALLIE-REID F, VANDEN BERGHE W, VISSER K, BINDER A, AFRICANDER D, VISMER M, DE BOSSCHER K, HAPGOOD JP, HAEGEMAN G, LOUW A. Abrogation of Glucocorticoid Receptor Dimerization Correlates with Dissociated Glucocorticoid Behavior of Compound A. Journal of Biological Chemistry 2010; 285(11) : 8061-8075.
ROHWER JM, HOFMEYR J-HS. Kinetic and Thermodynamic Aspects of Enzyme Control and Regulation. Journal of Physical Chemistry B 2010; 114 : 16280-16289.
ROOTMAN I, DE VILLIERS M, BRAND LA, STRAUSS E. Creating Cellulose-Binding Domain Fusions of the Coenzyme A Biosynthetic Enzymes to Enable Reactor-Based Biotransformations. ChemCatChem 2010; 2 : 1239-1251.
SOMDYALA NIM, BRADSHAW D, GELDERBLOM WCA, PARKIN DM. Cancer incidence in a rural population of South Africa, 1998-2002. International Journal of Cancer 2010; 127 : 2420-2429.
STRAUSS E, DE VILLIERS M, ROOTMAN I. Biocatalytic Production of Coenzyme A Analogues.ChemCatChem 2010; 2 : 929-937.
STRAUSS E. Exploiting single-cell variation for new antibiotics. Nature Chemical Biology 2010; 6 : 873-874.
SULPICE R, TRENKAMP S, STEINFATH M, USADEL B, GIBON Y, WITUCKA-WALL H, PYL E-V, TSCHOEP H, STEINHAUSER MC, GUENTHER M, HOEHNE M, ROHWER JM, ALTMANN T, FERNIE AR, STITT M. Network Analysis of Enzyme Activities and Metabolite Levels and Their Relationship to Biomass in a Large Panel ofArabidopsis Accessions. Plant Cell 2010; 22 : 2872-2893.
SWART AC, STORBECK K-H, SWART P. A single amino acid residue, Ala 105, confers 16alpha-hydroxylase activity to human cytochrome P450 17alpha-hydroxylase/17,20 lyase. Journal of Steroid Biochemistry and Molecular Biology 2010; 119 : 112-120.
THEMBO KM, VISMER HF, NYAZEMA NZ, GELDERBLOM WCA, KATERERE DR. Antifungal activity of four weedy plant extracts against selected mycotoxigenic fungi. Journal of Applied Microbiology 2010; 109 : 1479-1486.
VAN DER MERWE JD, JOUBERT E, MANLEY M, DE BEER D, MALHERBE CJ, GELDERBLOM WCA. In vitro hepatic biotransformation of aspalathin and nothofagin, dihydrochalcones of rooibos (Aspalathus linearis), and assessment of metabolite antioxidant activity. Journal of Agricultural and Food Chemistry 2010; 58 : 2214-2220.
VAN DER WESTHUIZEN L, SHEPHARD GS, RHEEDER JP, BURGER H.-M., GELDERBLOM WCA, WILD CP, GONG YY. Simple intervention method to reduce fumonisin exposure in a subsistence maize-farming community in South Africa. Food Additives and Contaminants 2010; 27(11) : 1582-1588.
VAN DER WESTHUYZEN R, STRAUSS E. Michael Acceptor-Containing Coenzyme A Analogues As Inhibitors of the Atypical Coenzyme A Disulfide Reductase from Staphylococcus aureus. Journal of the American Chemical Society 2010; 132 : 12853-12855.
VISSER K, SMITH C, LOUW A. Interplay of the Inflammatory and Stress Systems in a Hepatic Cell Line: Interactions between Glucocorticoid Receptor Agonists and Interleukin-6. Endocrinology 2010; 151(11) : 5279-5293.
Journal Articles (non-subsidised)
SPRY C, VAN SCHALKWYK DA, STRAUSS E, SALIBA KJ. Pantothenate Utilization by Plasmodium as a Target for Antimalarial Chemotherapy. Infectious Disorders – Drug Targets 2010; 10 : 200-216.
Proceedings International
HOUGH D, STORBECK K-H, CLOETE SWP, SWART P. Improving stress coping ability: Comparison between the CYP17 genotypes of Ovis aries and Capra hircus. 9th World Congress on Genetics Applied to Livestock Production, Congress Centre,Leipzig,Germany, Gesellschaft fur Tierzuchtwissen-schafen e.V. (German Society for Animal Science) 2010: 10-15.
ROHWER JM, HOFMEYR J-HS. Using Generalised Supply-Demand Analysis to Identify Regulatory Metabolites. Experimental Standard Conditions of Enzyme Characterizations – Proceedings of the International Builsstein Symposium, Hotel Jagdschloss Niederwald, Rüdesheim/Rhein, Germany, Logos Verlag Berlin GmbH – ISBN978-3-8325-2606-1 2010: 149-163.
Chapters in Books
STRAUSS E. Coenzyme A Biosynthesis and Enzymology. In Comprehensive Natural Products II – Chemistry and Biology. Volume 7 of 10, Elsevier,Oxford,United Kingdom, 2010: 351-410.
???Doctorals completed?
?AFRICANDER D. Comparative study of the molecular mechanism of action of the synthetic progestins, Medroxyprogesterone acetate and Norethisterone acetate. PhD(Bioch), 2010. 113 pp. Promotor: Hapgood JP. Medepromotor: Louw A.
MALHERBE CJ. Control Analysis of Mixed Populations of Gluconobacter oxydans and Saccharomyces cerevisiae. PhD(Bioch), 2010. 123 pp. Promotor: Snoep JL. Medepromotor: Rohwer JM.
SPATHELF B. Qualitative structure-activity relationships of the major tyrocidines, cyclic decapeptides from Bacillus aneurinolyticus. PhD(Bioch), 2010. 206 pp. Promotor: Rautenbach M.?
Masters completed??
DU TOIT F. Control Analysis of the Action Potential and its Propagation in the Hodgkin-Huxley Model. MSc Biochemie, 2010. 93 pp. Studieleier: Snoep JL.
SMITH J. Experimental supply demand analysis of yeast fermentative free-energy metabolism: an in vivo and in situinvestigation. MSc Biochemie, 2010. 117 pp. Studieleier: Rohwer JM. Medestudieleier: Snoep JL.
STEENMANS S. The identification, contiguous sequence annotation, cloning and site direct mutagenesis of the P100 vaccine candidate gene of the ostrich mycoplasma Ms02. MSc Biochemie, 2010. 125 pp. Studieleier: Bellstedt DU. Medestudieleier:Botes A.
VISSER K. Effect of modulators of inflammation on hepatic Acute Phase Proteins and Metabolic Enzymes. MSc Biochemie, 2010. 148 pp. Studieleier: Louw A.??
